Zhang Yi-Da, Ma Chen, Shi Yan-Ping
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, PR China.
Gansu Province Key Laboratory of Evidence Science Techniques Research and Application, Gansu University of Political Science and Law, Lanzhou, PR China.
Anal Chim Acta. 2022 Dec 15;1236:340584. doi: 10.1016/j.aca.2022.340584. Epub 2022 Nov 4.
Simple and disposable monitoring of blood is usually the best solution for early clinical diagnosis and home self-inspection of the chronic patients. Herein, a simple point-of-care (POC) device which called molecularly imprinted membrane modified gel colorimetric device (MIMGCD) was prepared for whole blood cholesterol colorimetric detection at the first time. The convenient detection principle of this device rely on molecularly imprinted membranes for specifically separating cholesterol from whole blood firstly, following the gold bipyramids (GBPs) agarose gel system is react with the cholesterol oxidation to product hydrogen peroxide (HO), and the cholesterol will be quantified based on the color change. Under optimal conditions, the analytical performance of the proposed device yielded a linear range of 315.8-6000.0 μM and detection limit of 94.7 μM with 6.89% RSD for cholesterol, which can meet the needs of the detection of normal cholesterol content in the human body. Compared with the traditional whole blood detection methods, no complex sample preparation steps or precision instruments are required, endowing MIMGCD with the merits of easy to operate and low-cost. In addition, the multicolor variation of GBPs in the device allow a colorimetric card-like detection mechanism, which can be used for home self-inspection. This device has the potential to be utilized in clinical and home POC testing application for whole blood biomolecule analysis, thereby facilitating the whole blood screening and long-term monitoring in non-specialized laboratory infrastructure.
对于慢性病患者的早期临床诊断和家庭自我检查而言，简单且一次性使用的血液监测通常是最佳解决方案。在此，首次制备了一种名为分子印迹膜修饰凝胶比色装置（MIMGCD）的简单即时检测（POC）设备，用于全血胆固醇的比色检测。该设备便捷的检测原理是，首先依靠分子印迹膜从全血中特异性分离胆固醇，随后金双锥体（GBPs）琼脂糖凝胶体系与胆固醇氧化反应生成过氧化氢（HO），并根据颜色变化对胆固醇进行定量。在最佳条件下，所提出的设备对胆固醇的分析性能线性范围为315.8 - 6000.0 μM，检测限为94.7 μM，相对标准偏差（RSD）为6.89%，能够满足人体正常胆固醇含量检测的需求。与传统的全血检测方法相比，无需复杂的样品制备步骤或精密仪器，赋予了MIMGCD操作简便和成本低廉的优点。此外，该设备中GBPs的多色变化允许采用比色卡式检测机制，可用于家庭自我检查。该设备有潜力应用于临床和家庭POC检测中的全血生物分子分析，从而在非专业实验室基础设施中促进全血筛查和长期监测。
