Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Austria.
Centro de Investigaciones Sobre Desertificación (CIDE, CSIC-UV-GV), Carretera de Moncada-Náquera Km 4.5, 46113, Moncada, Valencia, Spain.
Commun Biol. 2022 Nov 17;5(1):1261. doi: 10.1038/s42003-022-04178-y.
Ectomycorrhizal fungi live in close association with their host plants and form complex interactions with bacterial/archaeal communities in soil. We investigated whether abundant or rare ectomycorrhizal fungi on root-tips of young beech trees (Fagus sylvatica) shape bacterial/archaeal communities. We sequenced 16S rRNA genes and fungal internal transcribed spacer regions of individual root-tips and used ecological networks to detect the tendency of certain assemblies of fungal and bacterial/archaeal taxa to inhabit the same root-tip (i.e. modularity). Individual ectomycorrhizal root-tips hosted distinct fungal communities associated with unique bacterial/archaeal communities. The structure of the fungal-bacterial/archaeal association was determined by both, dominant and rare fungi. Integrating our data in a conceptual framework suggests that the effect of rare fungi on the bacterial/archaeal communities of ectomycorrhizal root-tips contributes to assemblages of bacteria/archaea on root-tips. This highlights the potential impact of complex fine-scale interactions between root-tip associated fungi and other soil microorganisms for the ectomycorrhizal symbiosis.
外生菌根真菌与宿主植物密切相关,并与土壤中的细菌/古菌群落形成复杂的相互作用。我们研究了在年轻山毛榉树(Fagus sylvatica)根尖上丰富或稀有的外生菌根真菌是否会塑造细菌/古菌群落。我们对单个根尖的 16S rRNA 基因和真菌内转录间隔区进行了测序,并使用生态网络来检测某些真菌和细菌/古菌类群组合倾向于栖息在同一根尖上的趋势(即模块性)。单个外生菌根根尖上宿主的真菌群落不同,与独特的细菌/古菌群落相关。真菌-细菌/古菌的关联结构由优势真菌和稀有真菌共同决定。将我们的数据整合到一个概念框架中表明,稀有真菌对根际外生菌根真菌细菌/古菌群落的影响有助于根际细菌/古菌的组合。这突出了与根尖相关真菌和其他土壤微生物之间复杂的细尺度相互作用对外生菌根共生的潜在影响。