Han Xiaoxiang, Huang Jianghui, Cai Jinwang, Yu Chen, Jiang Shengchou, Ye Qing, Yang Shuifen, Pan Weichun, Zhang Qiaozhi, Wang Yanbo
College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou, China.
J Sci Food Agric. 2023 Apr;103(6):3006-3016. doi: 10.1002/jsfa.12343. Epub 2022 Dec 7.
As potent antioxidants, anthocyanins can protect the body from free radicals. However, the traditional solvent extraction method has the disadvantages of requiring a high extraction temperature and long extraction time, so it is necessary to develop an efficient extraction method for anthocyanins.
In this study, the technique of natural deep eutectic solvents (DESs) was applied to extract anthocyanins from purple perilla leaves with the aid of microwave-ultrasonic assisted extraction (MUAE). The response surface methodology (RSM), based on the Box-Behnken design (BBD), predicted the maximum extraction yield of anthocyanins to be 619.62 mg (100 g) under the following conditions: x (ultrasonic extraction power) = 357.25 W, x (time) = 25.62 min, and x (temperature) = 57.80 °C. The biological activity of the extract obtained was evaluated by examining its radical-scavenging effect on 1,1-diphenyl-2-picrylhydrazyl, hydroxyl radical, and superoxide anion radicals. Its bacteriostatic impact was investigated on four typical bacteria: Shewanella putrefaciens (S. putrefaciens), Pseudomonas fluorescens (P. fluorescens), Escherichia coli (E. coli), and Staphylococcus aureus (S. aureus).
The integrated extraction method of DESs with MUAE was efficient, energy-saving, green, and sustainable. © 2022 Society of Chemical Industry.
作为强效抗氧化剂，花青素可保护身体免受自由基侵害。然而，传统的溶剂萃取法存在萃取温度高、时间长的缺点，因此有必要开发一种高效的花青素萃取方法。
本研究采用天然深共熔溶剂（DESs）技术，借助微波 - 超声辅助萃取（MUAE）从紫苏叶中提取花青素。基于Box - Behnken设计（BBD）的响应面法（RSM）预测，在以下条件下花青素的最大萃取产率为619.62毫克/（100克）：x（超声萃取功率）= 357.25瓦，x（时间）= 25.62分钟，x（温度）= 57.80℃。通过检测提取物对1,1 - 二苯基 - 2 - 苦基肼自由基、羟基自由基和超氧阴离子自由基的清除作用，评估所得提取物的生物活性。研究了其对四种典型细菌的抑菌作用：腐败希瓦氏菌（S. putrefaciens）、荧光假单胞菌（P. fluorescens）、大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）。
DESs与MUAE的集成萃取方法高效、节能、绿色且可持续。© 2022化学工业协会。
