Khudhur Zhikal O, Othman Goran, Othman Galawezh O, Jafaar Aziz M, Qadir Mahdi Kh, Awla Harem Kh, Qasim Sara Sh, Hadi Shayma M, Khan Samiha S, Noreen Sobia, Smail Shukur W
Department of Biology, Faculty of Education, Tishk International University - Erbil, Kurdistan Region, Iraq.
Department of Medical Laboratory Technology, Erbil Health and Medical Technical College, Erbil Polytechnic University, Erbil, Kurdistan Region, Iraq.
J Taibah Univ Med Sci. 2022 Sep 5;18(1):162-169. doi: 10.1016/j.jtumed.2022.08.006. eCollection 2023 Feb.
The genetic polymorphisms of the endothelial nitric oxide synthase () gene are strongly associated with several cardiovascular diseases (CVDs) in various populations. The current study aimed to investigate the association of the rs1800779 (A/G) polymorphism with the progress of myocardial infarction (MI).
Eighty-five healthy subjects and 80 patients with MI admitted to the Erbil Cardiac Centre in the Kurdistan Region of Iraq were enrolled in the study. All participants were Kurdish from the same ethnic group. The amplification refractory mutation system polymerase chain reaction (ARMS-PCR) was used to determine the rs1800779 (A/G) polymorphism of , and the nitric oxide (NO) serum level was detected by spectrophotometer.
The genotypic frequencies of the rs1800779 AA (wild type), AG, and GG were 58.75%, 33.75%, and 7.50%, respectively, in the MI patients, and 49.41%, 43.53%, and 7.06%, respectively, for the control group. The frequencies of the A and the G alleles were 75.6% and 24.4%, respectively, in the MI group, and 71.2% and 28.8%, respectively, in the control subjects. The results revealed a lack of association of the rs1800779 genotype distribution with the level of NO serum and increased risk of MI.
The study concluded that there is a lack of association between the genotypes and alleles of the rs1800779 and susceptibility to MI in the studied population.
内皮型一氧化氮合酶（）基因的遗传多态性与不同人群中的多种心血管疾病（CVD）密切相关。本研究旨在探讨rs1800779（A/G）多态性与心肌梗死（MI）进展的关联。
伊拉克库尔德地区埃尔比勒心脏中心收治的85名健康受试者和80名MI患者纳入本研究。所有参与者均为同一族群的库尔德人。采用扩增阻滞突变系统聚合酶链反应（ARMS-PCR）测定的rs1800779（A/G）多态性，并用分光光度计检测血清一氧化氮（NO）水平。
MI患者中rs1800779 AA（野生型）、AG和GG的基因型频率分别为58.75%、33.75%和7.50%，对照组分别为49.41%、43.53%和7.06%。MI组中A和G等位基因的频率分别为75.6%和24.4%，对照组分别为71.2%和28.8%。结果显示rs1800779基因型分布与血清NO水平及MI风险增加之间缺乏关联。
该研究得出结论，在所研究人群中，rs1800779的基因型和等位基因与MI易感性之间缺乏关联。
