金雀异黄素通过肌苷-5'-单磷酸脱氢酶2/蛋白激酶B1途径促进肺癌细胞凋亡。
Genistein promotes apoptosis of lung cancer cells through the IMPDH2/AKT1 pathway.
作者信息
Xu Hao, Ma Hongda, Zha Lifen, Li Qian, Pan Huiming, Zhang Ladi
机构信息
Department of Respiratory Medicine, The People's Hospital of Danyang, Affiliated Danyang Hospital of Nantong University Danyang 212300, Jiangsu, P.R. China.
出版信息
Am J Transl Res. 2022 Oct 15;14(10):7040-7051. eCollection 2022.
OBJECTIVE
Lung cancer (LC) is a clinically challenging cancer. Genistein is a natural isoflavone product with anti-tumor effects. This study aims to investigate the effect of genistein on A549 cell apoptosis, to provide more experimental evidence for clinical treatment.
METHODS
Real-time quantitative polymerase chain reaction, western blotting, molecular docking, and target prediction methods were performed to detect the effect of genistein on LC cells. Cell viability of A549 treated by genistein was measured by a CCK-8 assay. The A549 cell apoptosis after genistein treatment was detected by flow cytometry.
RESULTS
Genistein promoted the apoptosis of LC cells in a time- and concentration-dependent manner. In addition, the low expression of inosine monophosphate dehydrogenase-2 (IMPDH2) inhibited the effect of genistein on LC cells. By predicting IMPDH2 LC-related apoptosis genes and finding the closely related gene protein kinase B (AKT1), it was found that the highly expressed AKT1 inhibited the effect of genistein on LC cell apoptosis and viability.
CONCLUSION
Genistein may be a promising treatment for LC.
目的
肺癌（LC）是一种具有临床挑战性的癌症。金雀异黄素是一种具有抗肿瘤作用的天然异黄酮产品。本研究旨在探讨金雀异黄素对A549细胞凋亡的影响，为临床治疗提供更多实验依据。
方法
采用实时定量聚合酶链反应、蛋白质免疫印迹法、分子对接和靶点预测方法检测金雀异黄素对肺癌细胞的作用。采用CCK-8法检测金雀异黄素处理后A549细胞的活力。采用流式细胞术检测金雀异黄素处理后A549细胞的凋亡情况。
结果
金雀异黄素以时间和浓度依赖性方式促进肺癌细胞凋亡。此外，肌苷单磷酸脱氢酶-2（IMPDH2）的低表达抑制了金雀异黄素对肺癌细胞的作用。通过预测IMPDH2与肺癌相关的凋亡基因并找到密切相关的基因蛋白激酶B（AKT1），发现高表达的AKT1抑制了金雀异黄素对肺癌细胞凋亡和活力的作用。
结论
金雀异黄素可能是一种有前景的肺癌治疗药物。
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