Department of Materials Science and Engineering, National Taiwan University, Taipei, Da'an Dist., 106319, Taiwan.
Department of Ophthalmology, National Taiwan University College of Medicine, Taipei, Zhongzheng Dist., 100233, Taiwan.
Macromol Biosci. 2023 Feb;23(2):e2200286. doi: 10.1002/mabi.202200286. Epub 2022 Dec 7.
This paper reports dual-function (high cell attachment and cell viability) fibrous scaffolds featuring aligned fibers, displaying good biocompatibility and no cytotoxicity. These scaffolds are fabricated through the electrospinning of a co-polypeptide comprising molar equivalents of N -carbobenzyloxy-l-lysine and γ-benzyl-l-glutamate, with the lysine moieties enhancing cell adhesion and the neural-stimulating glutamate moieties improving cell viability. These new scaffolds allow neural cells to attach and grow effectively without any special surface treatment or coating. Pheochromocytoma (PC-12) cells grown on these scaffolds exhibit better neuronal activity and longer neurite length, relative to those grown on scaffolds prepared from their respective homo-polypeptides. When the scaffolds are partially hydrolyzed such that they present net positive charge and increased hydrophilicity, the cell viability and neurite growth both increase further. Accordingly, these novel co-polypeptide fibrous scaffolds have potential applications in neural tissue engineering.
本文报道了一种具有定向纤维的双重功能（高细胞黏附性和细胞活力）纤维支架，具有良好的生物相容性和无细胞毒性。这些支架是通过包含等摩尔当量的 N-苄氧羰基-L-赖氨酸和 γ-苄基-L-谷氨酸的共多肽的静电纺丝制备的，其中赖氨酸部分增强细胞黏附，神经刺激谷氨酸部分提高细胞活力。这些新支架允许神经细胞有效附着和生长，而无需任何特殊的表面处理或涂层。与在各自的均聚物支架上生长的细胞相比，在这些支架上生长的嗜铬细胞瘤（PC-12）细胞表现出更好的神经元活性和更长的神经突长度。当支架部分水解，呈现净正电荷和增加的亲水性时，细胞活力和神经突生长都会进一步增加。因此，这些新型共多肽纤维支架在神经组织工程中有潜在的应用。
