Wang Jia-Wei, Shang Kun, Wu Sheng-Yi, Zhu Bo-Yu, Han Mei-Gui, Zhao Yuan, Chen Yi-Qiu, Xie Jia, Huang Bo, Han Zhuang
Hainan University, Haikou, Hainan 570228, China; Sanya Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, Hainan 572000, China.
Sanya Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, Hainan 572000, China.
Mar Genomics. 2022 Dec;66:100996. doi: 10.1016/j.margen.2022.100996. Epub 2022 Nov 8.
Stutzerimonas kunmingensis 7850S is a piezotolerant bacterium isolated from the sediment of the Mariana trench. Here, we described the complete genome of strain 7850S, which contains a single circular chromosome of 4,775,870 base pairs with 62.66% G + C content, and harbors 4494 protein-coding genes, 65 transfer RNA genes, and 12 ribosomal RNA genes. The experimental results showed that strain 7850S could grow under hydrostatic pressure of 0.1-70 MPa. Genomic analyses led to identifications of numbers of high hydrostatic pressure-associated genes, such as the ones associated with unsaturated fatty acids, betaine, and ectoine. A complete set of denitrification genes and some heavy metal detoxification genes were also found in this strain. The presence of these genes suggests metabolic characteristics for adaption to hadal environments and provides insights to further understand adaption strategies and ecological roles of Stutzerimonas in hadal environments.
昆明施氏菌7850S是一种从马里亚纳海沟沉积物中分离出的耐压细菌。在此，我们描述了菌株7850S的完整基因组，其包含一条4775870个碱基对的单环染色体，G+C含量为62.66%，并含有4494个蛋白质编码基因、65个转运RNA基因和12个核糖体RNA基因。实验结果表明，菌株7850S能够在0.1-70MPa的静水压力下生长。基因组分析鉴定出了许多与高静水压力相关的基因，如与不饱和脂肪酸、甜菜碱和四氢嘧啶相关的基因。在该菌株中还发现了一套完整的反硝化基因和一些重金属解毒基因。这些基因的存在表明了其适应超深渊环境的代谢特征，并为进一步了解施氏菌在超深渊环境中的适应策略和生态作用提供了见解。
