Marine Biological Resources and Environment Center, MNR Key Lab for Science & Technology of Marine Ecosystems First Institute of Oceanography, Qingdao, Shandong 266061, China.
College of Chemical Engineering, Qingdao University of Science & Technology, Qingdao, Shandong 266042, China.
Mar Genomics. 2022 Dec;66:100995. doi: 10.1016/j.margen.2022.100995. Epub 2022 Oct 23.
Pseudomonas sp. strain DNDY-54, a denitrifying bacterium, was isolated from a deep-sea sediment sample from Ninety East Ridge in the Indian Ocean. Here, we show that the complete genome of DNDY-54 has one circular chromosome of 4,412,895 bp with mean 60.57% GC content. The complete genome contains 4111 predicted protein-coding genes, 59 tRNAs, and 4 rRNA operons as 16S-23S-5S rRNA. On the basis of the annotation results, we identified genes that encode 27 proteins related to nitrogen metabolism, including enzymes that make up a complete denitrifying pathway. This work will improve the understanding of nitrogen cycling in the deep biosphere and provides a new candidate for protection of the environment and applications in waste water disposal.
从印度洋东 90 脊深海沉积物样本中分离出一株脱氮菌，命名为 Pseudomonas sp. 菌株 DNDY-54。本研究展示了 DNDY-54 的完整基因组为一个大小为 4412895bp 的环形染色体，GC 含量平均为 60.57%。完整基因组包含 4111 个预测蛋白编码基因、59 个 tRNA 和 4 个 rRNA 操纵子（16S-23S-5S rRNA）。基于注释结果，我们鉴定了编码 27 种与氮代谢相关的蛋白质的基因，其中包括构成完整脱氮途径的酶。这项工作将增进对深海生物圈氮循环的理解，并为环境保护和废水处理应用提供了新的候选对象。
