School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei, Anhui 230009, China; Institute of Solid State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
Institute of Solid State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
J Hazard Mater. 2023 Feb 15;444(Pt A):130403. doi: 10.1016/j.jhazmat.2022.130403. Epub 2022 Nov 14.
Amoxicillin (AMO) is one of the most commonly used antibiotics, and its abuse in animal husbandry or clinical therapy can pose unpredictable hazards to humans. Therefore, it is crucial to develop a real-time and rapid method to accurately determine AMO content. Here, we designed a fluorescent nanoprobe for qualitative and quantitative AMO determination by using as-synthesized green safe materials of nontoxic red carbon dots (RCDs) and blue carbon dots (BCDs). In the presence of AMO, a reaction promoting hydrogen bonding occurred immediately, resulting in an instant increase in the intensity of the blue fluorescence of BCDs, accompanied by a marked color change from red to blue. For practical application, we designed a nontoxic sensing fluorescent handy needle to directly and quantitatively detect AMO in real samples. This portable and easy-to-use device was demonstrated on a smartphone platform based on 3D printing technology, which offers the advantages of simple production, excellent visualization, fast response, and instant quantitative detection. The device requires an extremely short detection time and has a sensitive detection limit of 2.39 nM. The method presented here enables real-time assessment for food safety, as well as on-site detection under field conditions to track various trace substances for timely health checks.
阿莫西林（AMO）是最常用的抗生素之一，其在畜牧业或临床治疗中的滥用会对人类造成不可预测的危害。因此，开发一种实时、快速的方法来准确测定 AMO 的含量至关重要。在这里，我们设计了一种荧光纳米探针，用于定性和定量测定 AMO，该探针使用合成的无毒绿色材料——红色碳点（RCDs）和蓝色碳点（BCDs）。在 AMO 的存在下，立即发生了促进氢键形成的反应，导致 BCDs 的蓝色荧光强度瞬间增加，同时颜色从红色显著变为蓝色。为了实际应用，我们设计了一种无毒感应荧光便捷针，可直接定量检测实际样品中的 AMO。该便携式、易于使用的设备是基于 3D 打印技术在智能手机平台上展示的，具有制作简单、可视化效果好、响应快速和即时定量检测等优点。该设备的检测时间极短，检测限低至 2.39 nM。该方法可用于实时评估食品安全，以及现场条件下的现场检测，以跟踪各种痕量物质，进行及时的健康检查。
