Shi Fengming, Yu Tao, Xu Yabei, Zhang Sainan, Niu Yiming, Ge Sixun, Tao Jing, Zong Shixiang
Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China.
Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, Guiyang University, Guiyang 550005, China.
Int J Biol Macromol. 2023 Jan 15;225:634-647. doi: 10.1016/j.ijbiomac.2022.11.125. Epub 2022 Nov 17.
The genus Monochamus within the subfamily Lamiinae is the main vector of Bursaphelenchus xylophilus, which causes pine wilt disease and induces substantial economic and ecological losses. Only three complete mitochondrial genomes of the genus Monochamus have been sequenced to date, and no comparative mitochondrial genomic studies of Lamiinae have been conducted. Here, the mitochondrial genomes of two Monochamus species, M. saltuarius and M. urussovi, were newly sequenced and annotated. The composition and order of genes in the mitochondrial genomes of Monochamus species are conserved. All transfer RNAs exhibit the typical clover-leaf secondary structure, with the exception of trnS1. Similar to other longhorn beetles, Lamiinae mitochondrial genomes have an A + T bias. All 13 protein-coding genes have experienced purifying selection, and tandem repeat sequences are abundant in the A + T-rich region. Phylogenetic analyses revealed congruent topologies among trees inferred from the five datasets, with the monophyly of Acanthocinini, Agapanthiini, Batocerini, Dorcaschematini, Pteropliini, and Saperdini receiving high support. The findings of this study enhance our understanding of mitochondrial genome evolution and will provide a basis for future studies of population genetics and phylogenetic investigations in this group.
沟胫天牛亚科中的墨天牛属是松材线虫的主要传播媒介,松材线虫可引发松材线虫病,造成巨大的经济和生态损失。迄今为止,仅对墨天牛属的三个线粒体基因组进行了测序,尚未开展沟胫天牛亚科的线粒体基因组比较研究。在此,新测序并注释了两种墨天牛(盐泽墨天牛和乌苏里墨天牛)的线粒体基因组。墨天牛属线粒体基因组中基因的组成和顺序是保守的。除trnS1外,所有转运RNA均呈现典型的三叶草二级结构。与其他天牛类似,沟胫天牛亚科的线粒体基因组具有A+T偏好性。所有13个蛋白质编码基因都经历了纯化选择,并且在富含A+T的区域串联重复序列丰富。系统发育分析表明,从五个数据集推断出的树之间具有一致的拓扑结构,棘胫天牛族、阿加潘天牛族、白条天牛族、多卡天牛族、木棉天牛族和楔天牛族的单系性得到了高度支持。本研究结果增进了我们对线粒体基因组进化的理解,并将为该类群未来的群体遗传学研究和系统发育研究提供基础。