Martha Orendu Oche Attah, Tamunotonye Watson Jacks, Sani Hyedima Garba, Sadiya Ufeli Balogun
Department of Human Anatomy, College of Basic Medical Sciences, University of Maiduguri, P.M.B. 1069, Maiduguri, Borno State Nigeria.
Department of Medicine, Faculty of Medicine, Cyprus International University, Nicosia, Cyprus.
J Diabetes Metab Disord. 2022 Aug 3;21(2):1273-1281. doi: 10.1007/s40200-022-01017-z. eCollection 2022 Dec.
: Diabetes Mellitus is a major health problem characterized by hyperglycemia and disturbances in metabolism and implicated in causing oxidative stress. Treatment includes administration of oral hypoglycaemic agents with lifestyle modifications, these offer glycemic control, however, present limitations about availability, affordability and side effects. Traditional anti-diabetic plants are becoming popular in management of diabetes mellitus. This study was carried out to determine the efficacy of in treatment of diabetes.
Diabetes mellitus was induced in using a single injection of streptozotocin (50 mg kg i.p.). The rats were divided into four groups of 5 rats each. Groups 3-6 received olive oil, 100 mg kg extract, 200 mg.kg extract and insulin (6IU kg), respectively. 10 non-diabetic rats were grouped into two group receiving olive oil and 200 mg kg extract for 28 days. All groups were sacrificed by injecting with ketamine hydrochloride, blood was collected by cardiac puncture and centrifuged. The serum was analyzed for biochemical parameters. The liver was removed and homogenized with the supernatant of the resultant homogenate collected and used for analysis of oxidative stress enzymes.
The extract significantly decreased serum AST (p < 0.05), ALP (p < 0.001), ALT (p < 0.05), TG (p < 0.01), TC (p < 0.001), creatinine (p < 0.001). It had no effect on SOD and CAT levels but it significantly increased (p < 0.001) GSH levels and reduced (p < 0.05) MDA level.
The n-hexane extract of significantly decreased the levels of hepatic and renal serum biomarkers proving that it was beneficial in ameliorating diabetic related complications. The extract significantly increased GSH levels and reduced MDA level.
The online version contains supplementary material available at 10.1007/s40200-022-01017-z.
糖尿病是一个主要的健康问题，其特征为高血糖以及代谢紊乱，并与氧化应激的产生有关。治疗方法包括使用口服降糖药并改变生活方式，这些方法可实现血糖控制，然而，在可用性、可负担性和副作用方面存在局限性。传统抗糖尿病植物在糖尿病管理中越来越受欢迎。本研究旨在确定[具体植物名称]提取物在治疗糖尿病方面的疗效。
通过单次腹腔注射链脲佐菌素（50mg/kg）诱导大鼠患糖尿病。将大鼠分为四组，每组5只。第3 - 6组分别接受橄榄油、100mg/kg提取物、200mg/kg提取物和胰岛素（6IU/kg）。将10只非糖尿病大鼠分为两组，分别接受橄榄油和200mg/kg提取物，持续28天。所有组通过注射盐酸氯胺酮处死，通过心脏穿刺收集血液并离心。分析血清中的生化参数。取出肝脏并匀浆，收集所得匀浆的上清液用于分析氧化应激酶。
该提取物显著降低了血清天冬氨酸转氨酶（AST，p < 0.05）、碱性磷酸酶（ALP，p < 0.001）、丙氨酸转氨酶（ALT，p < 0.05）、甘油三酯（TG，p < 0.01）、总胆固醇（TC，p < 0.001）、肌酐（p < 0.001）水平。它对超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平没有影响，但显著提高了（p < 0.001）谷胱甘肽（GSH）水平并降低了（p < 0.05）丙二醛（MDA）水平。
[具体植物名称]的正己烷提取物显著降低了肝脏和肾脏血清生物标志物的水平，证明其有助于改善糖尿病相关并发症。该提取物显著提高了GSH水平并降低了MDA水平。
在线版本包含可在10.1007/s40200 - 022 - 01017 - z获取的补充材料。
