Vosti K L
J Med Microbiol. 1978 Nov;11(4):453-62. doi: 10.1099/00222615-11-4-453.
Purified M proteins were recovered from acid extracts of Streptococcus pyogenes, M-types 1, 3, 6, 12 and 17, by elution from columns of hydroxyapatite of the proteins precipitated with ammonium sulphate. M protein free from non-type-specific antigens was recovered only from M-type 12. Although similar fractions were not recovered from M-types 1, 3, 6 and 17, purified preparations containing a single cross-reactive antigen were obtained. In addition to the M proteins associated with cross-reactive antigens, type-specific antigens that did not stimulate opsonic antibodies were isolated from revealed molecular weights that ranged from 32,000 to 63,000 daltons, total amino acid compositions that were similar, and N-terminal amino acids that were variable.
从化脓性链球菌M1、M3、M6、M12和M17型的酸提取物中，通过从用硫酸铵沉淀的蛋白质的羟基磷灰石柱上洗脱，回收纯化的M蛋白。仅从M12型中回收了不含非型特异性抗原的M蛋白。虽然从M1、M3、M6和M17型中未回收类似的组分，但获得了含有单一交叉反应抗原的纯化制剂。除了与交叉反应抗原相关的M蛋白外，还从揭示的分子量范围为32,000至63,000道尔顿、总氨基酸组成相似且N端氨基酸可变的物质中分离出不刺激调理素抗体的型特异性抗原。
