Zhang Qi, Jia Shiliang, Ding Yicheng, Li Dongmei, Ding Yuting, Zhou Xuxia
College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, China.
Key Laboratory of Marine Fishery Resources Exploitment and Utilization of Zhejiang Province, Hangzhou, China.
Front Microbiol. 2022 Nov 1;13:979388. doi: 10.3389/fmicb.2022.979388. eCollection 2022.
Malondialdehyde (MDA) is one of the most representative reactive carbonyl species (RCSs) produced by lipid oxidation in food. However, the inhibitory effect of MDA on microorganisms has received little attention. Thus, the aim of this study was to reveal the antibacterial mechanism of MDA on and isolated from dry-cured fish. The results showed that the minimum inhibitory concentrations (MICs) of MDA on and were 90 μg/ml and 180 μg/ml, respectively. Time-kill curves indicated a concentration-dependent antibacterial activity of MDA. Moreover, cell wall damage, cell membrane depolarization, intracellular adenosine triphosphate (ATP) decline, Ca and Mg leakage, cell morphological destruction and alterations in intracellular biomolecules were observed, which indicated the negative influence of MDA on cell membrane and cellular homeostasis. This study demonstrated the potential antimicrobial properties of MDA and provided theoretical support for the scientific prevention and control of lipid oxidation and microbial contamination in food. This study demonstrated the potential antibacterial properties of MDA and further enriches theoretical studies on the effects of lipid oxidation on microorganisms.
丙二醛（MDA）是食品中脂质氧化产生的最具代表性的活性羰基化合物（RCSs）之一。然而，MDA对微生物的抑制作用鲜受关注。因此，本研究的目的是揭示MDA对从咸鱼中分离出的[具体微生物名称1]和[具体微生物名称2]的抗菌机制。结果表明，MDA对[具体微生物名称1]和[具体微生物名称2]的最低抑菌浓度（MICs）分别为90μg/ml和180μg/ml。时间-杀菌曲线表明MDA具有浓度依赖性抗菌活性。此外，还观察到细胞壁损伤、细胞膜去极化、细胞内三磷酸腺苷（ATP）含量下降、钙和镁泄漏、细胞形态破坏以及细胞内生物分子的变化，这表明MDA对细胞膜和细胞内稳态有负面影响。本研究证明了MDA潜在的抗菌特性，为科学防控食品中的脂质氧化和微生物污染提供了理论支持。本研究证明了MDA潜在的抗菌特性，并进一步丰富了脂质氧化对微生物影响的理论研究。
