Ghanem Heba Bassiony, El-Sharkawy Rehab Galal, Taha Rania Hosny
Clinical laboratory sciences Department, College of Applied Medical Sciences, Jouf University, Sakaka, Aljouf2014, Saudi Arabia.
Medical Biochemistry Department, Faculty of Medicine, Tanta University, Tanta31527, Egypt.
ACS Omega. 2022 Nov 4;7(45):41627-41640. doi: 10.1021/acsomega.2c05631. eCollection 2022 Nov 15.
Nanotechnology is the study of materials' unique properties at the nanoscale. Nanomedicine is the application of nanotechnology in medicine, which has been utilized to treat some common diseases, such as cancer. The of the present work is to synthesize the cadmium (Cd) nanocomplex using paracetamol as a ligand with a molar ratio of 1:2 M/L that was characterized by different physicochemical methods and to explore the effect of the synthesized Cd nanocomplex on the immune system and the redox status of the body and their anticancer effects on Ehrlich ascites carcinoma (EAC) induced in mice. Eighty female albino mice were separated into control; EAC; EAC treated with a low-dose Cd nanocomplex; and EAC treated with a high-dose Cd nanocomplex. Interleukin-6 (IL-6), NLR family pyrin domain containing 3 (NLRP3), and 8-hydroxy 2-deoxyguanosine (8-OHdG) levels were assessed by enzyme-linked immunosorbent assay (ELISA). Peroxynitrite level and glutathione peroxidase activity were assessed by spectrophotometry. NRF2 mRNA expression, cadmium content, and liver and renal toxicity were estimated. There was a significant increase in IL-6, NLRP3, 8-OHdG, peroxynitrite, and NRF2 mRNA expressions and in the glutathione peroxidase activity in EAC treated with low- and high-dose Cd nanocomplexes. However, the EAC treated with high-dose Cd nanocomplex group showed significant liver and renal toxicity. Cadmium nanocomplex has anticancer effects on EAC induced in mice via on the immune system and redox status as well as pyroptosis and epigenetic instability of the body, while high doses of Cd nanocomplex can cause liver and renal toxicity.
纳米技术是研究材料在纳米尺度下的独特性质。纳米医学是纳米技术在医学中的应用，已被用于治疗一些常见疾病，如癌症。本研究的目的是使用对乙酰氨基酚作为配体，以1:2 M/L的摩尔比合成镉（Cd）纳米复合物，并用不同的物理化学方法对其进行表征，并探究合成的Cd纳米复合物对免疫系统和机体氧化还原状态的影响及其对小鼠诱发的艾氏腹水癌（EAC）的抗癌作用。80只雌性白化小鼠被分为四组：对照组；EAC组；低剂量Cd纳米复合物治疗的EAC组；高剂量Cd纳米复合物治疗的EAC组。通过酶联免疫吸附测定（ELISA）评估白细胞介素-6（IL-6）、含NLR家族吡咯结构域3（NLRP3）和8-羟基-2-脱氧鸟苷（8-OHdG）水平。通过分光光度法评估过氧亚硝酸盐水平和谷胱甘肽过氧化物酶活性。估计NRF2 mRNA表达、镉含量以及肝脏和肾脏毒性。低剂量和高剂量Cd纳米复合物治疗的EAC组中，IL-6、NLRP3、8-OHdG、过氧亚硝酸盐和NRF2 mRNA表达以及谷胱甘肽过氧化物酶活性均显著增加。然而，高剂量Cd纳米复合物治疗的EAC组显示出显著的肝脏和肾脏毒性。镉纳米复合物通过影响机体的免疫系统和氧化还原状态以及细胞焦亡和表观遗传不稳定性，对小鼠诱发的EAC具有抗癌作用，而高剂量的Cd纳米复合物可导致肝脏和肾脏毒性。
