Soubeiga Adama Patrice, Kpoda Dissinviel Stéphane, Compaoré Muller K A, Somda-Belemlougri Asseto, Kaseko Ndamiwe, Rouamba Sibiri Sylvain, Ouedraogo Sandrine, Traoré Roukiatou, Karfo Paulette, Nezien Désiré, Nikiéma Fulbert, Kabre Elie, Zongo Cheikna, Savadogo Aly
Applied Biochemistry and Immunology Laboratory (LABIA), Sciences and Technologies Doctoral School, Joseph KI-ZERBO University, 03 BP 7021 03, Ouagadougou 03, Burkina Faso.
National Public Health Laboratory, 09 BP 24, Ouagadougou 09, Burkina Faso.
Int J Microbiol. 2022 Nov 9;2022:9640828. doi: 10.1155/2022/9640828. eCollection 2022.
The emergence of antimicrobial-resistantfood-borne bacteria is a great challenge to public health. This study was conducted to characterize and determine the resistance profile of Salmonella strains isolated from foods including sesames, ready-to-eat (RTE) salads, mango juices, and lettuce in Burkina Faso. One hundred and forty-eight biochemically identified Salmonella isolates were characterized by molecular amplification of Salmonella marker and , , , and virulence genes. After that, all confirmed strains were examined for susceptibility to sixteen antimicrobials, and PCR amplifications were used to identify the following resistance genes: , , , , , , , , and . One hundred and eight isolates were genetically confirmed as spp. Virulence genes were observed in 57.4%, 55.6%, 49.1%, and 38% isolates for , , , and , respectively. Isolates have shown moderate resistance to gentamycin (26.8%), ampicillin (22.2%), cefoxitin (19.4%), and nalidixic acid (18.5%). All isolates were sensitive to six antibiotics, including cefotaxime, ceftazidime, aztreonam, imipenem, meropenem, and ciprofloxacin. Among the 66 isolates resistant to at least one antibiotic, 11 (16.7%) were multidrug resistant. The Multiple Antimicrobial Resistance (MAR) index of Salmonella serovars ranged from 0.06 to 0.53. PCR detected 7 resistance genes (, , , , , , and ) in drug-resistant isolates. These findings raise serious concerns because ready-to-eat food in Burkina Faso could serve as a reservoir for spreading antimicrobial resistance genes worldwide.
抗菌性食源细菌的出现对公共卫生构成了巨大挑战。本研究旨在对从布基纳法索的芝麻、即食(RTE)沙拉、芒果汁和生菜等食品中分离出的沙门氏菌菌株进行特征分析,并确定其耐药性概况。通过对沙门氏菌标记物以及、、和毒力基因进行分子扩增,对148株经生化鉴定的沙门氏菌分离株进行了特征分析。之后,对所有确认的菌株进行了16种抗菌药物的敏感性检测,并使用聚合酶链反应(PCR)扩增来鉴定以下耐药基因:、、、、、、、和。108株分离株经基因确认为沙门氏菌属。分别在57.4%、55.6%、49.1%和38%的分离株中观察到、、和毒力基因。分离株对庆大霉素(26.8%)、氨苄青霉素(22.2%)、头孢西丁(19.4%)和萘啶酸(18.5%)表现出中度耐药性。所有分离株对六种抗生素敏感,包括头孢噻肟、头孢他啶、氨曲南、亚胺培南、美罗培南和环丙沙星。在对至少一种抗生素耐药的66株分离株中,有11株(16.7%)为多重耐药。沙门氏菌血清型的多重抗菌耐药(MAR)指数范围为0.06至0.53。PCR在耐药分离株中检测到7种耐药基因(、、、、、和)。这些发现引发了严重关注,因为布基纳法索的即食食品可能成为全球传播抗菌耐药基因的储存库。