College of Dentistry, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq.
College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq.
Open Vet J. 2024 Jul;14(7):1689-1700. doi: 10.5455/OVJ.2024.v14.i7.18. Epub 2024 Jul 31.
Food safety and food-borne infections are major subjects of global interest. Dairy products are considered as important source for these infections.
The present study was conducted to identify the occurrence and to genotype isolates of spp. recovered from milk samples in Al-Diwaniyah City, Iraq.
The current study included the collection of 50 milk samples purchased from local stores in the current city. These samples were subjected to bacterial cultivation and biochemical tests. Later, the growth was used to extract the genomic DNA that was exposed to PCR and partial gene sequencing both targeted the gene at a specific genetic piece.
The PCR results demonstrated the amplification of the genetic fragment of five genetic clusters for each of (SAD11, SAD12, SAD13, SAD14, and SAD15), (SED1, SED2, SED3, SED4, and SED5), and (SID1, SID2, SID3, SID4, and SID5). The PCR products were sent out to sequencing and reported that the current isolates were similar in their genetic content with global isolates at 95.34% to 97.59%, 96.21% to 97.57%, and 96.09% to 97.88%, respectively, of identity.
The present findings show high genetic variations among isolates of , , and recovered from milk samples, and these genotypes are found in different infection settings related to humans and animals, which may pose high risks to humans and animals.
食品安全和食源性感染是全球关注的主要课题。乳制品被认为是这些感染的重要来源。
本研究旨在鉴定从伊拉克迪瓦尼亚市牛奶样本中分离出的 spp 的发生情况和基因型。
本研究包括收集 50 份来自当前城市当地商店的牛奶样本。这些样本进行细菌培养和生化试验。随后，使用生长物提取基因组 DNA，然后对其进行 PCR 和部分基因测序，针对特定遗传片段的 基因。
PCR 结果显示，五个遗传簇的基因片段均得到了扩增，分别为 （SAD11、SAD12、SAD13、SAD14 和 SAD15）、 （SED1、SED2、SED3、SED4 和 SED5）和 （SID1、SID2、SID3、SID4 和 SID5）。将 PCR 产物送测序，并报告称，目前的分离株与全球分离株的遗传内容相似，相似度分别为 95.34%至 97.59%、96.21%至 97.57%和 96.09%至 97.88%。
本研究结果表明，从牛奶样本中分离出的 、 和 的分离株具有很高的遗传变异，这些基因型存在于与人类和动物相关的不同感染环境中，这可能对人类和动物构成高风险。
