Parisay Iman, Qeidari Ali, Sabouri Ehsan, Khorakian Fatemeh, Bagheri Hosein
Department of Pediatric Dentistry, Dental Material Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Molecular Medicine, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Dent Res J (Isfahan). 2022 Sep 26;19:79. eCollection 2022.
This study aims to compare cytotoxicity and induced apoptosis of a new bioceramic cement containing different concentrations of simvastatin on stem cells from human exfoliated deciduous teeth (SHED).
This research was an study. To evaluate the cytotoxicity and induced apoptosis of the bioceramic cement containing different concentrations of simvastatin, the SHED were exposed to the cement during 1, 3, and 7 days. Pure bioceramic cement and pure simvastatin with concentrations of 1, 0.1, and 0.01 μM were also tested to evaluate the possible synergic effect. Mineral trioxide aggregate (MTA) as the gold standard of pulp dressing materials was compared. MTT assay and Annexin V assay were used to evaluate cytotoxicity and induced apoptosis, respectively. The data were analyzed using ANOVA and Tukey tests at the significance level of 0.05.
During 7 days, MTA, bioceramic cement, simvastatin 0.1 and 0.01 μM, and bioceramic cement containing 0.1 and 0.01 μM simvastatin increased ( < 0.05) and simvastatin with concentration of 1 μM decreased the cell viability ( < 0.05). Except for MTA and bioceramic cement containing 0.1 and 0.01 μM simvastatin, all other compounds induced apoptosis within 7 days ( < 0.05).
After 7 days, the viability of the SHED in the presence of a new bioceramic cement containing 0.1 and 0.01 μM simvastatin was not compromised. Moreover, this cement showed superior results than MTA and provided an environment for cell proliferation. This finding appears to be due to the pharmacological effects of low concentrations of simvastatin.
本研究旨在比较含不同浓度辛伐他汀的新型生物陶瓷水泥对人乳牙脱落干细胞（SHED）的细胞毒性和诱导凋亡作用。
本研究为一项[具体研究类型未给出]研究。为评估含不同浓度辛伐他汀的生物陶瓷水泥的细胞毒性和诱导凋亡作用，将SHED暴露于该水泥中1天、3天和7天。还测试了纯生物陶瓷水泥和浓度为1、0.1和0.01μM的纯辛伐他汀，以评估可能的协同作用。将矿物三氧化物凝聚体（MTA）作为牙髓敷料材料的金标准进行比较。分别采用MTT法和膜联蛋白V法评估细胞毒性和诱导凋亡。数据采用方差分析和Tukey检验进行分析，显著性水平为0.05。
在7天内，MTA、生物陶瓷水泥、0.1和0.01μM的辛伐他汀以及含0.1和0.01μM辛伐他汀的生物陶瓷水泥使细胞活力增加（P<0.05），而浓度为1μM的辛伐他汀使细胞活力降低（P<0.05）。除MTA和含0.1和0.01μM辛伐他汀的生物陶瓷水泥外，所有其他化合物在7天内均诱导凋亡（P<0.05）。
7天后，在含0.1和0.01μM辛伐他汀的新型生物陶瓷水泥存在下，SHED的活力未受影响。此外，这种水泥显示出比MTA更好的结果，并为细胞增殖提供了环境。这一发现似乎归因于低浓度辛伐他汀的药理作用。
