Maru Viral, Madkaikar Manisha, Gada Ashita, Pakhmode Vivek, Padawe Dimple, Bapat Salil
Department of Pediatric Dentistry, Government Dental College and Hospital, Mumbai, Maharashtra, India.
Director, ICMR - National Institute of Immunohematology, Mumbai, Maharashtra, India.
Dent Res J (Isfahan). 2023 Apr 26;20:55. eCollection 2023.
The aim of this study was to investigate and compare the cytotoxicity and gene expression of Bio-C Repair, Mineral Trioxide Aggregate (MTA) HP Repair, and Biodentine on stem cells derived from exfoliated deciduous teeth.
In this study MTT assay was used to assess the cellular viability at three different dilutions. The gene expression of Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteocalcin [OCN], and dentin matrix protein-1 (DMP-1) was measured with real-time polymerase chain reaction after 7 days, 14 days, and 21 days of incubation. One-way analysis of variance and Bonferroni posttest were used for statistical analysis (p=o.o5).
After 72 h of incubation at dilution 1:4, stem cells derived from human exfoliated deciduous teeth (SHEDs) cultivated in Biodentine, followed by Bio-C Repair and MTA Repair HP reported with highest cellular viability. The highest mRNA expression of Runx2, ALP, OCN, and DMP-1 was reported in SHEDs cultured in Biodentine (after 21 days of incubation).
Bio-C Repair and MTA HP Repair are biocompatible and capable of odontogenic differentiation similar to Biodentine when cultured in stem cells derived from exfoliated primary teeth.
本研究旨在调查和比较生物C修复材料、高纯度矿物三氧化物凝聚体（MTA HP修复材料）和生物活性玻璃陶瓷对乳牙脱落干细胞的细胞毒性和基因表达。
在本研究中，采用MTT法评估三种不同稀释度下的细胞活力。在培养7天、14天和21天后，通过实时聚合酶链反应测量 runt相关转录因子2（Runx2）、碱性磷酸酶（ALP）、骨钙素[OCN]和牙本质基质蛋白-1（DMP-1）的基因表达。采用单因素方差分析和Bonferroni事后检验进行统计分析（p = 0.05）。
在1:4稀释度下孵育72小时后，在生物活性玻璃陶瓷中培养的乳牙脱落干细胞（SHEDs），其次是生物C修复材料和MTA HP修复材料，细胞活力最高。在生物活性玻璃陶瓷中培养的SHEDs（孵育21天后）中，Runx2、ALP、OCN和DMP-1的mRNA表达最高。
当在乳牙脱落干细胞中培养时，生物C修复材料和MTA HP修复材料具有生物相容性，并且能够与生物活性玻璃陶瓷一样进行牙源性分化。
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