Wang Li, Shao Li, Chen Man-Yun, Wang Lin, Yang Pu, Tan Feng-Bo, Zhang Wei, Huang Wei-Hua
Department of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Xiangya Hospital, Central South University, Changsha, Hunan 410078, P. R. China.
Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics Xiangya Hospital, Central South University, Changsha, Hunan 410078, P. R. China.
Am J Chin Med. 2023;51(1):107-127. doi: 10.1142/S0192415X23500076. Epub 2022 Nov 21.
Gut microbiota are significantly associated with the occurrence and development of inflammatory bowel disease (IBD). saponins (PNS) could be used for colitis and to modulate gut microbiota. However, the mechanism behind the effects of PNS on anti-colitis that are pertinent to gut microbiota is largely unknown. This study aimed to evaluate the anti-colitis effects of PNS and explore the involved mechanism as it is related to gut microbiota. Results showed that PNS significantly alleviated dextran sulfate sodium (DSS)-induced colitis. Meanwhile, after PNS treatment, the tight junction proteins were enhanced and proinflammatory cytokines, such as TNF-[Formula: see text], IL-6, IL-1[Formula: see text], and IL-17, were decreased. Furthermore, were significantly increased after modeling, while PNS reduced their abundance and significantly increased the amount of . Importantly, and were correlated with the IBD disease indicators. Moreover, fecal microbiota transplantation (FMT) experiments confirmed that PNS-reshaped gut microbiota significantly alleviated DSS-induced colitis, while significantly reduced the levels of the LPS-induced cellular inflammatory factors IL-1[Formula: see text] and TNF-[Formula: see text]. In conclusion, PNS alleviated colitis pertinent to the upregulation of and downregulation of . in the gut.
肠道微生物群与炎症性肠病（IBD）的发生和发展密切相关。人参皂苷（PNS）可用于治疗结肠炎并调节肠道微生物群。然而，PNS对结肠炎的影响及其与肠道微生物群相关的机制在很大程度上尚不清楚。本研究旨在评估PNS的抗结肠炎作用，并探讨其与肠道微生物群相关的作用机制。结果表明，PNS显著减轻了葡聚糖硫酸钠（DSS）诱导的结肠炎。同时，PNS治疗后，紧密连接蛋白增强，促炎细胞因子如肿瘤坏死因子-[公式：见正文]、白细胞介素-6、白细胞介素-1[公式：见正文]和白细胞介素-17减少。此外，建模后[具体指标]显著增加，而PNS降低了它们的丰度并显著增加了[另一具体指标]的量。重要的是，[具体指标]与IBD疾病指标相关。此外，粪便微生物群移植（FMT）实验证实，PNS重塑的肠道微生物群显著减轻了DSS诱导的结肠炎，而[具体物质]显著降低了脂多糖诱导的细胞炎症因子白细胞介素-1[公式：见正文]和肿瘤坏死因子-[公式：见正文]的水平。总之，PNS通过上调肠道中的[具体指标]和下调[具体指标]减轻了结肠炎。
