氨基酸平衡饮食通过缓解炎症和调节肠道微生物群改善了 DSS 诱导的结肠炎。
Amino acid-balanced diets improved DSS-induced colitis by alleviating inflammation and regulating gut microbiota.
机构信息
School of Health Science and Engineering, University of Shanghai for Science and Technology, 516, Jungong Road, Shanghai, 200093, China.
National Grain Industry (Urban Grain and Oil Security) Technology Innovation Center, Shanghai, 200093, China.
出版信息
Eur J Nutr. 2022 Oct;61(7):3531-3543. doi: 10.1007/s00394-022-02906-y. Epub 2022 May 26.
PURPOSE
Inflammatory bowel disease (IBD) is a multifactorial chronic disease of the gastrointestinal tract. Dietary intervention in the treatment of IBD has gradually attracted more attention. In this study, amino acid-balanced diets (AABD) based on grains were developed and their influences on the regulation of IBD were investigated.
METHODS
Dextran sodium sulfate (DSS)-induced acute colitis mice model was employed to evaluate the effects of AABD. Pathological symptoms, intestinal inflammation, gut barrier proteins and gut microbiota were determined after AABD intake.
RESULTS
It was shown that AABD alleviated the symptoms of colitis by reducing the histological scores of mice colon, suppressing the expression of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) and upregulating the expression of tight junction proteins. Analysis of gut microbiota revealed that AABD altered the structure of gut microbiota by decreasing the abundance and richness of harmful bacteria induced by DSS (Escherichia-Shigella, Parasutterella, etc.) and increasing that of beneficial bacteria (Akkermansia, etc.). Correlation analysis indicated that the alterations of pro-inflammatory factors were related with the change of microbiota, suggesting that the inhibitory effects of AABD on inflammation might be due to its regulation gut microbiota.
CONCLUSION
The AABD could efficiently mitigate colitis, and this study indicated that AABD could be applied as a promising dietary regulation strategy of IBD.
目的
炎症性肠病（IBD）是一种多因素的胃肠道慢性疾病。饮食干预在 IBD 的治疗中逐渐受到更多关注。本研究开发了基于谷物的氨基酸平衡饮食（AABD），并研究了其对 IBD 调节的影响。
方法
采用葡聚糖硫酸钠（DSS）诱导的急性结肠炎小鼠模型来评估 AABD 的作用。摄入 AABD 后，测定病理症状、肠道炎症、肠道屏障蛋白和肠道微生物群。
结果
结果表明，AABD 通过降低小鼠结肠的组织学评分、抑制促炎细胞因子（IL-1β、IL-6 和 TNF-α）的表达和上调紧密连接蛋白的表达，减轻了结肠炎的症状。肠道微生物群分析表明，AABD 通过减少 DSS 诱导的有害细菌（如大肠杆菌-志贺氏菌、Parasutterella 等）的丰度和丰富度，增加有益细菌（如 Akkermansia 等），改变了肠道微生物群的结构。相关性分析表明，促炎因子的变化与微生物群的变化有关，这表明 AABD 对炎症的抑制作用可能与其调节肠道微生物群有关。
结论
AABD 可以有效缓解结肠炎，本研究表明 AABD 可以作为一种有前途的 IBD 饮食调节策略。
Zotero 插件