Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China.
Food Funct. 2022 Dec 13;13(24):12707-12720. doi: 10.1039/d2fo02577a.
Fermented egg-milk peptides (FEMPs) could alleviate the symptoms of inflammatory diseases but the underlying regulating mechanism of effective ingredients is unclear now. Our research was designed to confirm the protective function of FEMP, then analyze the potential targets and pathways that could be regulated by digested FEMP (dFEMP). The results showed that FEMP could ease the inflammatory symptoms in the colon, repair the damage of inflammation, and decrease the level of pro-inflammatory cytokines (decreased by 31.81% TNF-α, 60.20% IL-1β, 85.65% IL-6). The results of experiments revealed that dFEMP could influence many inflammation-related targets. Most targets affected the inflammation-related function and participated in the inflammatory signaling pathways, such as the T cell receptor (TCR) signaling pathway. Besides, molecular docking results revealed that hydrogen-bonding and salt bridges played vital roles in the dFEMP-target interactions. Combining experiments with experiments, this study can prove a new theory for research between the bioactive peptides and inflammation.
发酵蛋乳肽（FEMPs）可以缓解炎症性疾病的症状，但目前有效成分的潜在调节机制尚不清楚。我们的研究旨在确认 FEMP 的保护功能，然后分析可被消化的 FEMP（dFEMP）调节的潜在靶点和途径。结果表明，FEMP 可以缓解结肠的炎症症状，修复炎症损伤，并降低促炎细胞因子的水平（TNF-α 降低 31.81%，IL-1β 降低 60.20%，IL-6 降低 85.65%）。实验结果表明，dFEMP 可以影响许多与炎症相关的靶点。大多数靶点影响与炎症相关的功能，并参与炎症信号通路，如 T 细胞受体（TCR）信号通路。此外，分子对接结果表明氢键和盐桥在 dFEMP-靶标相互作用中起着至关重要的作用。将实验与实验相结合，本研究可以为生物活性肽与炎症之间的研究提供一个新的理论。
