School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China.
School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China.
J Ethnopharmacol. 2023 Jun 12;309:115935. doi: 10.1016/j.jep.2022.115935. Epub 2022 Nov 19.
ErZhiFormula (EZF) is a classical traditional Chinese medicinal formulation. It can be used to treat liver and kidney yin deficiency, dizziness, lumbar debility, insomnia, nocturnal emission, lower extremity weakness, and other aging-related diseases. However, the protective effect of EZF in skin photoaging and its potential mechanism has not been clarified.
This study aims to explore the role of EZF in the skin photoaging mechanism induced by UV radiation.
Ultra Performance Liquid Chromatography (UPLC) was used to identify the fingerprint of EZF. The mice were irradiated with UVA and UVB to establish the photoaging model in vivo. Human immortalized keratinocytes (HaCaT) were irradiated with UVB to establish the photoaging model in vitro. The activity of cells was detected by CCK-8 and LDH kits, the level of reactive oxygen species was detected by DCF fluorescent probe, and the apoptosis was detected by PE annexin V and 7-Amino-Actinomycin (7-AAD) staining. Comet assay was used to detect cell DNA damage. The antioxidant enzyme levels in cell and mouse serum were detected by antioxidant kit, and Western blot was used to detect protein expression.
We found that EZF contain many active ingredients, including salidroside, specnuezhenide, isoquercitrin, etc. EZF can improve the photoaging of HaCaT cells and mouse skin caused by UV radiation. The results of animal experiments are consistent with those of cell experiments. Combined with Western blot analysis, we found that EZF finally played an anti-skin photoaging role by regulating the Nrf2/HO-1/NQO1 pathway.
EZF can protect skin from UV-induced photoaging by regulating the Nrf2/HO-1/NQO1 signal pathway. EZF may become a traditional Chinese medicine with the potential to prevent skin photoaging.
二至丸（EZF）是一种经典的中药方剂。它可用于治疗肝肾阴虚、头晕、腰膝酸软、失眠、遗精、下肢无力和其他与衰老相关的疾病。然而，EZF 对皮肤光老化的保护作用及其潜在机制尚未阐明。
本研究旨在探讨 EZF 在 UVA 和 UVB 辐射诱导的皮肤光老化机制中的作用。
采用超高效液相色谱法（UPLC）鉴定 EZF 的指纹图谱。通过 UVA 和 UVB 照射建立体内光老化模型，通过 UVB 照射建立体外光老化模型。采用 CCK-8 和 LDH 试剂盒检测细胞活力，DCF 荧光探针检测活性氧水平，PE Annexin V 和 7-Amino-Actinomycin（7-AAD）染色检测细胞凋亡。彗星试验检测细胞 DNA 损伤。采用抗氧化试剂盒检测细胞和小鼠血清中抗氧化酶水平，采用 Western blot 检测蛋白表达。
我们发现 EZF 含有许多活性成分，包括红景天苷、獐牙菜苦苷、异槲皮苷等。EZF 可改善 UV 辐射引起的 HaCaT 细胞和小鼠皮肤的光老化。动物实验结果与细胞实验结果一致。结合 Western blot 分析，我们发现 EZF 最终通过调节 Nrf2/HO-1/NQO1 通路发挥抗皮肤光老化作用。
EZF 可通过调节 Nrf2/HO-1/NQO1 信号通路保护皮肤免受 UV 诱导的光老化。EZF 可能成为一种具有预防皮肤光老化潜力的中药。
