School of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA.
School of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA.
Trends Genet. 2023 Feb;39(2):154-166. doi: 10.1016/j.tig.2022.10.007. Epub 2022 Nov 20.
Gene-editing technologies have revolutionized the field of mosquito sensory biology. These technologies have been used to knock in reporter genes in-frame with neuronal genes and tag specific mosquito neurons to detect their activities using binary expression systems. Despite these advances, novel tools still need to be developed to elucidate the transmission of olfactory signals from the periphery to the brain. Here, we propose the development of a set of tools, including novel driver lines as well as sensors of neuromodulatory activities, which can advance our knowledge of how sensory input triggers behavioral outputs. This information can change our understanding of mosquito neurobiology and lead to the development of strategies for mosquito behavioral manipulation to reduce bites and disease transmission.
基因编辑技术彻底改变了蚊子感觉生物学领域。这些技术已被用于将报告基因与神经元基因进行框内敲入，并使用二元表达系统标记特定的蚊子神经元以检测其活性。尽管取得了这些进展，但仍需要开发新的工具来阐明从外围到大脑的嗅觉信号的传递。在这里，我们提出了一组工具的开发，包括新的驱动线以及神经调节活性传感器，这可以增进我们对感觉输入如何触发行为输出的理解。这些信息可以改变我们对蚊子神经生物学的理解，并导致开发蚊子行为操纵策略以减少叮咬和疾病传播。
