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荧光杂交自动化的现状与展望——实验室主任及其指定人员的调查。

Status and Prospects of Fluorescence Hybridization Automation-A Survey among Laboratory Directors and Their Designates.

机构信息

Cytogenetic Technology Program, School of Health Professions, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Department of Hematopathology, Division of Pathology and Laboratory Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

出版信息

Genes (Basel). 2022 Nov 11;13(11):2098. doi: 10.3390/genes13112098.

Abstract

As a clinical diagnostic technique, fluorescence hybridization (FISH) is simple, reliable, cost-effective and widely applicable. Due to technology advances, automation systems are adapted in FISH in different ways, involving all and/or some of the following procedural steps: sample processing, probe distribution, hybridization, post-wash, result analysis and/or final report preparation. To better understand the status and prospective of FISH automation, a survey has been recently performed among Cytogenetic Laboratory Directors and/or their designated Laboratory Managers, Supervisors or certified Cytogenetic Technologists. We present here the preliminary analysis of this survey, to advocate more discussion about standardization of the FISH automation as well as implementation of FISH automation as part of educational programs for Cytogenetic Technologists.

摘要

作为一种临床诊断技术,荧光原位杂交(FISH)简单、可靠、具有成本效益,并且适用范围广泛。由于技术的进步,自动化系统以不同的方式应用于 FISH,涉及所有和/或部分以下操作步骤:样本处理、探针分配、杂交、洗涤后处理、结果分析和/或最终报告准备。为了更好地了解 FISH 自动化的现状和前景,最近对细胞遗传实验室主任和/或其指定的实验室经理、主管或认证细胞遗传技术人员进行了一项调查。我们在此介绍该调查的初步分析,以提倡更多地讨论 FISH 自动化的标准化以及将 FISH 自动化作为细胞遗传技术人员教育计划的一部分进行实施。

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