Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, 2800, Kgs. Lyngby, Denmark.
Biomed Microdevices. 2012 Jun;14(3):453-60. doi: 10.1007/s10544-011-9622-7.
Cytogenetic and molecular cytogenetic analyses, which aim to detect chromosome abnormalities, are routinely performed in cytogenetic laboratories all over the world. Traditional cytogenetic studies are performed by analyzing the banding pattern of chromosomes, and are complemented by molecular cytogenetic techniques such as fluorescent in situ hybridization (FISH). To improve FISH application in cytogenetic analysis the issues with long experimental time, high volumes of expensive reagents and requirement for trained technicians need to be addressed. The protocol has recently evolved towards on chip detection of chromosome abnormalities with the development of microsystems for FISH analysis. The challenges addressed by the developed microsystems are mainly the automation of the assay performance, reduction in probe volume, as well as reduction of assay time. The recent focus on the development of automated systems for performing FISH on chip is summarized in this review.
细胞遗传学和分子细胞遗传学分析旨在检测染色体异常，目前在世界各地的细胞遗传学实验室中常规进行。传统的细胞遗传学研究通过分析染色体的带型模式进行，并辅以分子细胞遗传学技术，如荧光原位杂交（FISH）。为了改进 FISH 在细胞遗传学分析中的应用，需要解决实验时间长、昂贵试剂用量大以及对技术人员的要求等问题。随着用于 FISH 分析的微系统的发展，该方案最近已朝着芯片上染色体异常的检测方向发展。所开发的微系统解决的主要挑战是测定性能的自动化、探针体积的减少以及测定时间的减少。本文综述了最近在开发用于芯片上执行 FISH 的自动化系统方面的进展。
