Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan, 030006, China.
School of Science, Hainan University, Renmin Road 58, Haikou, 570228, China.
Adv Sci (Weinh). 2023 Jan;10(3):e2205080. doi: 10.1002/advs.202205080. Epub 2022 Nov 24.
The signal-to-noise ratio (SNR) is one of the key features of a fluorescent probe and one that often defines its potential utility for in vivo labeling and analyte detection applications. Here, it is reported that introducing a pyridine group into traditional cyanine-7 dyes in an asymmetric manner provides a series of tunable NIR fluorescent dyes (Cy-Mu-7) characterized by enhanced Stokes shifts (≈230 nm) compared to the parent cyanine 7 dye (<25 nm). The observed Stokes shift increase is ascribed to symmetry breaking of the Cy-Mu-7 core and a reduction in the extent of conjugation. The fluorescence signals of the Cy-Mu-7 dyes are enhanced upon confinement within the hydrophobic cavity of albumin or via spontaneous encapsulation within micelles in aqueous media. Utilizing the Cy-Mu-7, ultra-fast in vivo kidney labeling in mice is realized, and it is found that the liver injury will aggravate the burden of kidney by monitoring the fluorescence intensity ratio of kidney to liver. In addition, Cy-Mu-7 could be used as efficient chemiluminescence resonance energy transfer acceptor for the reaction between H O and bisoxalate. The potential utility of Cy-Mu-7 is illustrated via direct monitoring fluctuations in endogenous H O levels in a mouse model to mimic emergency room trauma.
信噪比（SNR）是荧光探针的关键特征之一，通常决定了其在体内标记和分析物检测应用中的潜在用途。本文报道了一种方法，即将吡啶基团以不对称的方式引入到传统的菁染料中，得到了一系列可调谐的近红外荧光染料（Cy-Mu-7），与母体菁染料（<25nm）相比，其斯托克斯位移（≈230nm）显著增加。观察到的斯托克斯位移增加归因于 Cy-Mu-7 核心的对称性破缺和共轭程度的降低。在白蛋白的疏水腔体内或在水相介质中自组装成胶束时，Cy-Mu-7 染料的荧光信号得到增强。利用 Cy-Mu-7，实现了超快速的体内肾脏标记，通过监测肾脏与肝脏的荧光强度比值发现，肝损伤会加重肾脏的负担。此外，Cy-Mu-7 可用作 H O 和双草酸酯反应的高效化学发光共振能量转移受体。通过直接监测小鼠模型中内源性 H O 水平的波动来模拟急诊创伤，说明了 Cy-Mu-7 的潜在用途。
