Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and iChEM, Fudan University, Shanghai, 200433, P. R. China.
School of Pharmacy, Fudan University, Shanghai, 200433, P. R. China.
Angew Chem Int Ed Engl. 2021 Jul 19;60(30):16294-16308. doi: 10.1002/anie.202007040. Epub 2021 Jan 19.
The development of fluorophores for the second near-infrared window (NIR-II, 1000-1700 nm) represents an emerging, significant, and vibrant field in analytic chemistry, chemical biology, and biomedical engineering. The wavelength, brightness, and stability are three crucial factors that determine the performance of an NIR-II fluorophore. Up to now, significant progress has been made in the development of NIR-II fluorescence molecular probes, including the synthesis of D-A-D and D-π-A fluorophores with improved NIR-II fluorescence imaging performance and the construction of off-on probes and ratiometric probes via energy transfer or molecular structure modification. In this review, we summarize the most recent advances in molecular engineering design strategies of NIR-II fluorophores and probes, then highlight a selection of bioimaging and biosensing applications. We also provide perspectives on potential challenges and opportunities in this emerging field.
用于近红外二区（NIR-II，1000-1700nm）的荧光团的发展在分析化学、化学生物学和生物医学工程领域是一个新兴的、重要的和充满活力的领域。波长、亮度和稳定性是决定近红外二区荧光团性能的三个关键因素。到目前为止，在开发 NIR-II 荧光分子探针方面已经取得了重大进展，包括通过能量转移或分子结构修饰合成具有改善的 NIR-II 荧光成像性能的 D-A-D 和 D-π-A 荧光团，以及构建开-关探针和比率探针。在这篇综述中，我们总结了 NIR-II 荧光团和探针的分子工程设计策略的最新进展，然后重点介绍了一些生物成像和生物传感应用。我们还对这个新兴领域的潜在挑战和机遇提供了一些看法。
