Zhang Qiang, Dou Wei, He Li-Qiang, Yu Shan-Shan, Chen Jia-Qing, Zheng Li-Yuan, Wang Lin, Smagghe Guy, Wang Jin-Jun
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Pest Manag Sci. 2023 Apr;79(4):1352-1361. doi: 10.1002/ps.7305. Epub 2022 Dec 8.
Most arthropods are famous for their large reproductive capacity, with the ovary playing a vital role in the process. The study of the regulatory mechanisms of ovarian development may have the potential for a reproduction-based pest management strategy. GATA-binding transcription factors (GATAs) as important regulatory factors mediate many physiological processes, including development, immunity, insecticide resistance and reproduction. The Pannier (pnr), a member of GATA family, was confirmed to be involved in ovarian development of Bactrocera dorsalis in our previous study. However, the direct evidence of pnr regulating the fly ovarian development is still lacking.
We used CRISPR/Cas9 to create Bdpnr loss-of-function mutations. Homozygous Bdpnr mutants were nonviable, with most individuals dying during embryogenesis, some surviving to the larval stages, and the remaining few dying during pupation. In contrast, heterozygous individuals reached the adult stage, but ovarian development was disrupted, with concomitant decreases in egg laying and hatching rates. We also found that two genes encoding vitellogenin proteins (BdVg1 and BdVg2) and the vitellogenin receptor (BdVgR) were significantly down-regulated in heterozygous mutants compared to wild-type controls.
These results indicate that Bdpnr is required for embryonic and post-embryonic development, including the formation of ovaries. Bdpnr could therefore be considered as a molecular target for tephritid fly pest control. © 2022 Society of Chemical Industry.
大多数节肢动物以其巨大的繁殖能力而闻名，卵巢在这一过程中起着至关重要的作用。研究卵巢发育的调控机制可能为基于生殖的害虫管理策略提供潜在依据。GATA结合转录因子（GATAs）作为重要的调控因子，介导许多生理过程，包括发育、免疫、抗药性和繁殖。在我们之前的研究中，已证实GATA家族成员Pannier（pnr）参与了橘小实蝇的卵巢发育。然而，仍缺乏pnr调控果蝇卵巢发育的直接证据。
我们使用CRISPR/Cas9技术创建了Bdpnr功能缺失突变体。纯合Bdpnr突变体无法存活，大多数个体在胚胎发育过程中死亡，一些存活到幼虫阶段，其余少数在化蛹时死亡。相比之下，杂合个体能够发育到成虫阶段，但卵巢发育受到破坏，产卵率和孵化率随之降低。我们还发现，与野生型对照相比，杂合突变体中两个编码卵黄原蛋白的基因（BdVg1和BdVg2）以及卵黄原蛋白受体（BdVgR）显著下调。
这些结果表明，Bdpnr是胚胎发育和胚后发育所必需的，包括卵巢的形成。因此，Bdpnr可被视为实蝇类害虫防治的分子靶点。© 2022化学工业协会。
