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可喷涂的 RNAi 用于沉默重要基因以管理红棕象甲，Rhynchophorus ferrugineus（鞘翅目：象甲科）。

Sprayable RNAi for silencing of important genes to manage red palm weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae).

机构信息

Central Laboratories, King Faisal University, Al-Ahsa, Saudi Arabia.

Institute of Plant Protection, MNS-University of Agriculture Multan, Multan, Pakistan.

出版信息

PLoS One. 2024 Oct 24;19(10):e0308613. doi: 10.1371/journal.pone.0308613. eCollection 2024.

DOI:10.1371/journal.pone.0308613
PMID:39446907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11501036/
Abstract

The red palm weevil, Rhynchophorus ferrugineus (Oliver, 1970) (Coleoptera: Dryophthoridae) is the most devastating insect-pest of palm trees worldwide. Synthetic insecticides are the most preferred tool for the management of RPW. Alternatively, RNA interference (RNAi) mediated silencing of crucial genes provides reasonable control of insect pests. Recently, we have targeted four important genes; ecdysone receptor (EcR), serine carboxypeptidase (SCP), actin and chitin-binding peritrophin (CBP) in the 3rd and 5th instar larvae RPW. The results from 20 days trial showed that the survival rate of 3rd instar larvae fed on SCP and actin dsRNAs exhibited the lowest survival (12-68%). While, in the 5th instar larvae, the lowest survival rate (24%) was recorded for SCP after 20 days of incubation. Similarly, the weight of the 3rd and 5th instar larvae treated with SCP and actin was significantly reduced to 2.30-2.36 g and 4.64-4.78 g after 6 days of dsRNA exposure. The larval duration was also decreased significantly in the larvae treated with all the dsRNA treatments. The qRT-PCR results confirmed a significant suppression of the targeted genes as 90-97% and 85-93% in the 3rd and 5th instar larvae, respectively. The results suggest that the SCP and the actin genes can be promising targets to mediate RNAi-based control of RPW.

摘要

红棕象甲，Rhynchophorus ferrugineus（Oliver，1970）（鞘翅目：象甲科）是世界范围内棕榈树最具破坏性的昆虫害虫。合成杀虫剂是管理 RPW 的最首选工具。或者，RNA 干扰（RNAi）介导的关键基因沉默为害虫的合理控制提供了可能。最近，我们针对 4 个重要基因，蜕皮激素受体（EcR）、丝氨酸羧肽酶（SCP）、肌动蛋白和几丁质结合围食膜蛋白（CBP），在 3 龄和 5 龄幼虫 RPW 中进行了靶向研究。20 天试验结果表明，饲喂 SCP 和肌动蛋白 dsRNA 的 3 龄幼虫存活率最低（12-68%）。而在 5 龄幼虫中，SCP 的存活率最低（24%），在孵育 20 天后记录到。同样，用 SCP 和肌动蛋白处理的 3 龄和 5 龄幼虫的体重在 6 天 dsRNA 暴露后分别显著减少到 2.30-2.36 g 和 4.64-4.78 g。所有 dsRNA 处理的幼虫的幼虫期也显著缩短。qRT-PCR 结果证实，在 3 龄和 5 龄幼虫中，目标基因的表达分别被显著抑制了 90-97%和 85-93%。结果表明，SCP 和肌动蛋白基因可能是介导红棕象甲基于 RNAi 的控制的有前途的靶标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/20f50f607cb2/pone.0308613.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/303ee0b65958/pone.0308613.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/cd81e2563f19/pone.0308613.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/233b782ec193/pone.0308613.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/ee8290cb22b7/pone.0308613.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/51c4cb195559/pone.0308613.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/20f50f607cb2/pone.0308613.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/303ee0b65958/pone.0308613.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/cd81e2563f19/pone.0308613.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/233b782ec193/pone.0308613.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/ee8290cb22b7/pone.0308613.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/51c4cb195559/pone.0308613.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bdd/11501036/20f50f607cb2/pone.0308613.g006.jpg

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