Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210016, China.
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Int J Mol Sci. 2022 Nov 18;23(22):14332. doi: 10.3390/ijms232214332.
Hy-Line Brown chickens’ health is closely related to poultry productivity and it is mainly maintained by the immune system, healthy intestinal function, and microflora of chicken. Polysaccharides are biological macromolecules with a variety of activities that can be used as a potential prebiotic to improve poultry health. In this experiment, the function of Alhagi honey polysaccharides (AH) as an immunomodulator on the chicken was investigated. All chicken (120) were randomly distributed to four groups (five replicas/group, six hens/replica). A total of 0.5 mL water was taken orally by the chicken in control group. AH (0.5 mL) in different concentrations (three dosages, 0.3 g/kg, 0.6 g/k, and 1.2 g/kg) were used for the AH-0.3 g/kg, AH-0.6 g/k, and AH-1.2 g/kg group, respectively. The results showed that the growth performance of the chickens and the index of immune organs (the weight of immune organs/the body weight) were enhanced significantly after being AH-treated (p < 0.05). The content of sIgA and cytokines was upregulated remarkably in the intestine after being AH-treated (p < 0.05). The AH treatment significantly enhanced the intestinal epithelial barrier (p < 0.05). Moreover, the percentage of CD4+ and CD8+ T cells in the ileum, spleen, and serum were obviously upscaled (p < 0.05). In addition, the AH treatment significantly enhanced the production of short chain fatty acids (SCFAs) and improved the structure of gut microbiota (p < 0.05). In conclusion, we found that AH-1.2g/kg was the best dosage to improve the chicken’s health, and these data demonstrated that AH could be used as a potential tool to enhance growth performance through improving intestine function, immunity, and gut microbiome in chicken.
苜蓿蜂蜜多糖对鸡的免疫调节作用
海兰褐鸡的健康状况与家禽的生产力密切相关，主要通过免疫系统、健康的肠道功能和鸡的微生物菌群来维持。多糖是具有多种活性的生物大分子，可以用作潜在的益生元来改善家禽的健康。本实验研究了苜蓿蜂蜜多糖（AH）作为免疫调节剂对鸡的作用。将 120 只鸡（每组 5 个重复，每个重复 6 只母鸡）随机分为四组。对照组鸡口服 0.5mL 水，AH 组分别用不同浓度（三种剂量，0.3g/kg、0.6g/k 和 1.2g/kg）的 0.5mLAH 处理，分别命名为 AH-0.3g/kg、AH-0.6g/k 和 AH-1.2g/kg。结果表明，AH 处理后，鸡的生长性能和免疫器官指数（免疫器官重量/体重）显著提高（p<0.05）。AH 处理后，肠道中 sIgA 和细胞因子的含量显著上调（p<0.05）。AH 处理显著增强了肠道上皮屏障（p<0.05）。此外，回肠、脾脏和血清中 CD4+和 CD8+T 细胞的百分比明显升高（p<0.05）。此外，AH 处理显著增加了短链脂肪酸（SCFAs）的产生，改善了肠道微生物群的结构（p<0.05）。综上所述，我们发现 AH-1.2g/kg 是改善鸡健康的最佳剂量，这些数据表明 AH 可以作为一种潜在的工具，通过改善肠道功能、免疫和肠道微生物群来提高鸡的生长性能。
