College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.
Int J Mol Sci. 2022 Nov 20;23(22):14435. doi: 10.3390/ijms232214435.
Glycoside hydrolases from pathogens have often been reported as inducers of immune responses. However, the roles of glycoside hydrolase from plant-growth-promoting rhizobacteria (PGPR) in the resistance of plants against pathogens is not well studied. In this study, we identified a glycoside hydrolase 43 protein, H1AD43, produced by BL06 that can trigger defense responses, including cell death. Ion-exchange and size-exclusion chromatography were used for separation, and the amino acid sequence was identified by mass spectrometry. The recombinant protein generated by prokaryotic expression was able to elicit a hypersensitive response (HR) in and trigger early defense responses, including reactive oxygen species (ROS) burst, callose accumulation, and the induction of defense genes. In addition, the protein could induce resistance in , in which it inhibited infection by Leonian and tobacco mosaic virus-green fluorescent protein (TMV-GFP) expression. H1AD43 thus represents a microbe-associated molecular pattern (MAMP) of PGPR that induces plant disease resistance and may provide a new method for the biological control of plant disease.
糖苷水解酶通常被报道为诱导免疫反应的物质。然而，植物促生根际细菌（PGPR）中的糖苷水解酶在植物抵抗病原体方面的作用尚未得到充分研究。在本研究中，我们鉴定了一株由 BL06 产生的糖苷水解酶 43 蛋白 H1AD43，它能够触发包括细胞死亡在内的防御反应。我们使用离子交换和大小排阻层析进行分离，并通过质谱鉴定其氨基酸序列。原核表达生成的重组蛋白能够在 中引发过敏反应（HR），并触发早期防御反应，包括活性氧（ROS）爆发、胼胝质积累和防御基因的诱导。此外，该蛋白还能够在 中诱导抗性，抑制 Leonian 和烟草花叶病毒-绿色荧光蛋白（TMV-GFP）的感染。因此，H1AD43 代表了一种能够诱导植物抗病性的 PGPR 相关分子模式（MAMP），可能为植物病害的生物防治提供一种新方法。
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