Korean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeollabuk-do, 56212, Republic of Korea.
Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Yuseong, Daejeon, 34113, Republic of Korea.
Sci Rep. 2024 Jul 9;14(1):15830. doi: 10.1038/s41598-024-66202-x.
Demequina, commonly found in coastal and marine environments, represents a genus of Actinomycetes. In this study, strains Demequina PMTSA13 and OYTSA14 were isolated from the rhizosphere of Capsicum annuum, leading to the discovery of a novel species, Demequina capsici. Bacteria play a significant role in plant growth, yet there have been no reports of the genus Demequina acting as plant growth-promoting bacteria (PGPB). Comparative genomics analysis revealed ANI similarity values of 74.05-80.63% for PMTSA13 and 74.02-80.54% for OYTSA14, in comparison to various Demequina species. The digital DNA-DNA hybridization (dDDH) values for PMTSA13 ranged from 19 to 39%, and 19.1-38.6% for OYTSA14. Genome annotation revealed the presence of genes associated with carbohydrate metabolism and transport, suggesting a potential role in nutrient cycling and availability for plants. These strains were notably rich in genes related to 'carbohydrate metabolism and transport (G)', according to their Cluster of Orthologous Groups (COG) classification. Additionally, both strains were capable of producing auxin (IAA) and exhibited enzymatic activities for cellulose degradation and catalase. Furthermore, PMTSA13 and OYTSA14 significantly induced the growth of Arabidopsis thaliana seedlings primarily attributed to their capacity to produce IAA, which plays a crucial role in stimulating plant growth and development. These findings shed light on the potential roles of Demequina strains in plant-microbe interactions and agricultural applications. The type strain is Demequina capsici PMTSA13 (= KCTC 59028 = GDMCC 1.4451), meanwhile OYTSA14 is identified as different strains of Demequina capsici.
德美卡菌通常存在于沿海和海洋环境中，是放线菌的一个属。在这项研究中，从辣椒的根际中分离出了菌株德美卡菌 PMTSA13 和 OYTSA14，从而发现了一个新物种，即辣椒德美卡菌。细菌在植物生长中起着重要作用，但尚未有关于德美卡菌作为植物促生菌（PGPB）的报道。比较基因组学分析显示，PMTSA13 和 OYTSA14 与各种德美卡菌的ANI 相似性值分别为 74.05-80.63%和 74.02-80.54%。PMTSA13 的数字 DNA-DNA 杂交（dDDH）值为 19-39%，OYTSA14 的 dDDH 值为 19.1-38.6%。基因组注释表明存在与碳水化合物代谢和转运相关的基因，这表明它们在植物的养分循环和可利用性方面可能发挥作用。根据它们的同源基因分类群（COG）分类，这些菌株富含与“碳水化合物代谢和转运（G）”相关的基因。此外，这两种菌株都能够产生吲哚乙酸（IAA），并且具有纤维素降解和过氧化氢酶的酶活性。此外，PMTSA13 和 OYTSA14 显著促进了拟南芥幼苗的生长，这主要归因于它们产生 IAA 的能力，IAA 在刺激植物生长和发育中起着关键作用。这些发现揭示了德美卡菌菌株在植物-微生物相互作用和农业应用中的潜在作用。模式菌株是辣椒德美卡菌 PMTSA13（=KCTC 59028=GDMCC 1.4451），同时 OYTSA14 被鉴定为不同的辣椒德美卡菌菌株。
