School of Biological & Chemical Engineering, Zhejiang University of Science & Technology, Hangzhou 310023, China.
Guizhou Academy of Tobacco Science, Guiyang 550001, China.
Molecules. 2022 Nov 17;27(22):7959. doi: 10.3390/molecules27227959.
The occurrence of crop fungal diseases is closely related to warm environmental conditions. In order to control the release of fungicides in response to warm conditions, and enhance the efficacy, a series of thermo-responsive fungicide-loaded nanoparticles were developed. The fungicide azoxystrobin, solvent DEP, emulsifier Tween 80 and thermo-responsive component TDA were combined to create thermal-response oil phases, conditions for emulsification were then optimized. LDLS, zeta potential, FTIR, DSC, TGA, XRD, SEM and antifungal efficacy assays were carried out to investigate the characteristics and forming mechanism. The results indicated that the formula with 5 g azoxystrobin, 10 mL DEP, 6 mL Tween 80 and 2.5 g TDA constructed the proposed oil phase with the ability to transform from solid at 20 °C to softerned at 31.5 °C. Both DEP and TDA played key roles in interfering with the crystallization of azoxystrobin. The optimal T3t-c12 nanoparticles had a mean particle size of 162.1 nm, thermo-responsive morphological transformation between 20 °C and 30 °C, AZO crystal reforming after drying, the ability to attach to fungal spores and satisfied antifungal efficacy against PNgz07 and A1513 at 30 °C. This report provides referable technical support for the construction of smart-release nanoparticles of other agrochemicals.
作物真菌病害的发生与温暖的环境条件密切相关。为了控制杀菌剂在温暖条件下的释放，并提高药效，开发了一系列温敏型载药纳米粒子。将杀菌剂嘧菌酯、溶剂二乙酯（DEP）、乳化剂吐温 80 和温敏组分 TDA 组合在一起，形成温敏油相，然后优化乳化条件。通过 LDLS、zeta 电位、FTIR、DSC、TGA、XRD、SEM 和抗真菌功效评估来研究其特性和形成机制。结果表明，含 5 g 嘧菌酯、10 mL DEP、6 mL 吐温 80 和 2.5 g TDA 的配方构建了拟议的油相，能够在 20°C 时从固态转变为 31.5°C 时的软化态。DEP 和 TDA 都在干扰嘧菌酯结晶方面发挥了关键作用。最优 T3t-c12 纳米粒子的平均粒径为 162.1nm，在 20°C 和 30°C 之间具有温敏形态转变，干燥后 AZO 晶体重构，能够附着在真菌孢子上，并在 30°C 时对 PNgz07 和 A1513 表现出满意的抗真菌功效。本报告为其他农药智能释放纳米粒子的构建提供了有价值的技术支持。
