Zhejiang Provincial Key Lab of Mariculture & Enhancement, Zhejiang Marine Fisheries Research Institute, Tiyu Road 28, Zhoushan 316021, China.
College of Food and Pharmacy, Zhejiang Ocean University, 1 South Haida Road, Zhoushan 316000, China.
Molecules. 2022 Nov 18;27(22):8012. doi: 10.3390/molecules27228012.
A water-soluble sulfated polysaccharide, F2-1, was obtained from the marine green alga, , using ion-exchange and size-exclusion chromatography. Structure analysis showed that the F2-1 was a sulfated arabinan comprising Ara, Rha, Man, Gal, and Xyl with an 18% sulfate content and a molecular weight of 100 kDa. Methylation analysis combined with desulfation, GC-MS, IR, and NMR spectroscopy showed that the backbone of F2-1 was →4)-β-L-Ara(1→ residue. Its 2- and/or 3- positions showed sulfate modification; additionally, the 2- or 3- position showed branch points. The side chains were composed of →5)-β-D-Gal, (1→2,6)-β-D-Gal(1→, (1→2)-β-L-Rha4S, →4)-α-D-Glc(1→, and terminal α-D-Gal(1→ and β-D-Xyl(1→. Polysaccharides containing β-D-galactofuranose are rarely found in seaweed. F2-1 exhibited significant anticoagulant activity in vitro. Our findings suggested that the green-tide alga, , can be considered as a useful resource for bioactive polysaccharides.
从海洋绿藻浒苔中提取到一种水溶性硫酸化多糖 F2-1，采用离子交换和凝胶过滤层析进行分离纯化。结构分析表明，F2-1 是一种由 Ara、Rha、Man、Gal 和 Xyl 组成的硫酸阿拉伯聚糖，硫酸基含量为 18%，分子量为 100 kDa。甲基化分析结合部分酸水解、GC-MS、IR 和 NMR 等波谱分析结果表明，F2-1 的主链由→4）-β-L-Ara（1→残基构成，其 2-和/或 3-位发生硫酸酯化修饰，此外，2-或 3-位还有支链点。侧链由→5）-β-D-Gal、（1→2,6）-β-D-Gal、（1→2）-β-L-Rha4S、→4）-α-D-Glc（1→和末端α-D-Gal（1→和β-D-Xyl（1→组成。在海藻中很少发现含有β-D-半乳糖呋喃糖的多糖。F2-1 在体外具有显著的抗凝活性。研究结果表明，绿潮藻浒苔可作为生物活性多糖的有用资源。
