Institute of Chemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
Hanoi Metropolitan University, Hanoi, Vietnam.
Nat Prod Res. 2024 Feb-Mar;38(4):555-562. doi: 10.1080/14786419.2023.2180506. Epub 2023 Feb 20.
In this article, chemical structure and conformation in an aqueous solution of a new sulfated polysaccharide, P, extracted from green seaweed were elucidated by SEC-MALL, IR, NMR and SAXS. The results indicated that the obtained polysaccharide is a sulfated arabinogalactan with a molecular weight of 223 kDa, and is mainly composed of →3,6)-α-D-Gal4S→ and →2)-α-L-Ara→ connecting together through 1→3 glycoside linkages. It has a broken rod-like conformation in solution with estimated as 0.43 nm from SAXS measurements. The polysaccharide exhibited a notable anticoagulant activity measured by the assays of activated partial thromboplastintime, thrombintime and prothrombine time as well as a significant cytotoxic activity against hepatocellular, human breast cancer, and cervical cancer cell lines.
本文通过 SEC-MALL、IR、NMR 和 SAXS 研究了从绿藻中提取的新型硫酸化多糖 P 在水溶液中的化学结构和构象。结果表明，所得多糖是一种硫酸阿拉伯半乳聚糖，分子量为 223kDa，主要由→3,6)-α-D-Gal4S→和→2)-α-L-Ara→通过 1→3 糖苷键连接而成。它在溶液中具有断裂的棒状构象，根据 SAXS 测量，其估计值为 0.43nm。该多糖具有显著的抗凝血活性，通过激活部分凝血活酶时间、凝血时间和凝血酶原时间测定，以及对肝癌、人乳腺癌和宫颈癌细胞系的显著细胞毒性活性。
