Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 102488, P. R. China.
School of Mongolian Materia Medica, Inner Mongolia University for Nationalities, Tongliao, 028000, P. R. China.
Chem Biodivers. 2023 Jan;20(1):e202200984. doi: 10.1002/cbdv.202200984. Epub 2022 Dec 7.
The heartwood of Syringa oblata Lindl. (SO) is one of Mongolian folk medicines to treat insomnia and pain, while its pharmacological evaluation and underlying mechanism remain unclear. In this study, the sedative effect of ethanol extract of SO (ESO) was evaluated with the locomotor activity test and the threshold dose of pentobarbital sodium-induced sleep test in mice, and the hot plate test, acetic acid-induced writhing test, and formalin test in mice were used to evaluate its analgesic effect. The underlying mechanism of ESO analgesia was explored by RT-PCR and western blot analysis, which is associated with the regulation of the NF-κB signaling pathway. Besides, the main constituents of ESO were characterized by LC/MS data analysis and comparison with isolated pure compounds. The current findings brought evidence for clinical application and further pharmacological and phytochemical studies on SO.
紫丁香的心材（SO）是蒙古民间医学用来治疗失眠和疼痛的药物之一，但其药理作用和作用机制尚不清楚。在这项研究中，通过小鼠的运动活性测试和戊巴比妥钠诱导的睡眠测试评价了紫丁香乙醇提取物（ESO）的镇静作用，并用热板测试、醋酸扭体测试和福尔马林测试评价了其镇痛作用。通过 RT-PCR 和 Western blot 分析探讨了 ESO 镇痛的作用机制，这与 NF-κB 信号通路的调节有关。此外，通过 LC/MS 数据分析和与分离的纯化合物进行比较，对 ESO 的主要成分进行了表征。目前的研究结果为 SO 的临床应用以及进一步的药理学和植物化学研究提供了证据。
