Department of Dentistry, State University of Ponta Grossa, Ponta Grossa, Brazil.
Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
Med Mycol. 2022 Nov 6;60(12). doi: 10.1093/mmy/myac092.
This study compared different conditions to establish a rat model of denture stomatitis. Immunocompetent Wistar rats were divided into two groups (n = 35): Tetracycline = administration of 0.83 mg/ml of tetracycline hydrochloride 7 days before induction of denture stomatitis and amoxicillin = administration of 0.156 mg/ml of amoxicillin with clavulanic acid 4 days before induction of denture stomatitis. A suspension of Candida albicans was inoculated on the palate followed by the use of a palatal device contaminated with C. albicans inoculum for 4 days to induce denture stomatitis. As controls, some rats were not submitted to any procedure or used a sterile palatal device for 4 days. The development of denture stomatitis was confirmed by visual analysis, colony-forming units per milliliter (CFU/ml) count, histopathological and immunohistochemical analyses, and through myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) assays. Rats were euthanized right after device removal (T0), 4 (T4), or 6 (T6) days after device removal. Tetracycline improved the development of the disease, with more severe clinical signs at T0. Similar results were observed in the CFU/ml count and in the histometric and immunohistochemical analyses. Higher MPO expression was detected in the palates of the tetracycline group (P = .006). Despite the subtle differences between antibiotics, tetracycline showed better results in inducing and maintaining denture stomatitis for at least 4 days after device removal.
本研究比较了不同条件，以建立义齿性口炎的大鼠模型。将免疫功能正常的 Wistar 大鼠分为两组（n=35）：四环素组=在诱导义齿性口炎前 7 天给予 0.83mg/ml 盐酸四环素，阿莫西林组=在诱导义齿性口炎前 4 天给予 0.156mg/ml 阿莫西林克拉维酸。将白色念珠菌混悬液接种于腭部，然后使用污染有白色念珠菌接种物的腭部装置 4 天以诱导义齿性口炎。作为对照，一些大鼠未进行任何处理或使用无菌腭部装置 4 天。通过肉眼分析、每毫升菌落形成单位（CFU/ml）计数、组织病理学和免疫组织化学分析以及髓过氧化物酶（MPO）和 N-乙酰氨基葡萄糖苷酶（NAG）测定来确认义齿性口炎的发展。大鼠在装置去除后立即（T0）、4 天（T4）或 6 天（T6）处死。四环素改善了疾病的发展，T0 时具有更严重的临床体征。CFU/ml 计数以及组织学和免疫组织化学分析也观察到了类似的结果。四环素组腭部 MPO 表达较高（P=0.006）。尽管抗生素之间存在细微差异，但四环素在诱导和维持义齿性口炎方面的效果更好，至少在装置去除后 4 天内如此。
