抗真菌剂牙托水疗在大鼠义齿性口炎模型中的疗效。

Effectiveness of a tissue conditioner containing antifungals in a rat model of denture stomatitis.

机构信息

Department of Dentistry, State University of Ponta Grossa, Ponta Grossa 84030-900, Brazil.

Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, Brazil.

出版信息

J Appl Microbiol. 2024 Jun 3;135(6). doi: 10.1093/jambio/lxae123.

DOI:10.1093/jambio/lxae123

PMID:38744659

Abstract

AIM

This study investigated the effectiveness of a drug-modified tissue conditioner in an animal model of denture stomatitis.

METHODS AND RESULTS

Wistar rats wore a Candida albicans-contaminated palatal device for 4 days. Next, nystatin (Nys) or chlorhexidine (Chx) were added to a tissue conditioner in their raw or β-cyclodextrin-complexed (βCD) forms at their minimum inhibitory concentrations. As controls, one group was not subjected to any procedure (NC), one group used sterile devices, one group had denture stomatitis but was not treated (DS), and another had the devices relined with the tissue conditioner without the addition of any drug (Soft). After 4 days of treatment, treatment effectiveness was assessed visually, histologically, and through CFU count, and myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) assays. Rats from the Soft, Nys, Nys:βCD, and Chx groups presented a significant decrease in the microbial load compared with the untreated group. Treatment groups showed lower MPO and NAG activity compared to the non-treated group.

CONCLUSIONS

The addition of antifungals to a soft tissue conditioner can be a promising approach for denture stomatitis treatment.

摘要

目的

本研究旨在动物模型中评估一种药物改性义齿基托组织面调节剂对义齿性口炎的治疗效果。

方法和结果

Wistar 大鼠佩戴被白色念珠菌污染的腭部装置 4 天。然后，在组织面调节剂中添加最低抑菌浓度的制霉菌素（Nys）或洗必泰（Chx），并分别以原始形式或 β-环糊精复合物（βCD）形式添加。作为对照，一组不进行任何处理（NC），一组使用无菌器械，一组患有义齿性口炎但未治疗（DS），另一组使用不含任何药物的组织面调节剂重新衬垫（Soft）。治疗 4 天后，通过肉眼观察、组织学观察和 CFU 计数以及髓过氧化物酶（MPO）和 N-乙酰氨基葡萄糖苷酶（NAG）测定评估治疗效果。与未治疗组相比，Soft、Nys、Nys:βCD 和 Chx 组大鼠的微生物负荷明显降低。与未治疗组相比，治疗组的 MPO 和 NAG 活性较低。