Yang Zhenxing, He Yuwen, Meng Jinxin, Li Nan, Wang Jinglin
Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Key Laboratory of Transboundary Animal Diseases Prevention and Control (Co-construction by Ministry and Province), Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan Province, China.
Virus Genes. 2023 Apr;59(2):223-233. doi: 10.1007/s11262-022-01959-9. Epub 2022 Nov 28.
In July 2019, a novel viral strain (JH2019C603) was isolated from sentinel cattle in Jinghong City, in the subtropical region of Yunnan Province, China. The virus replicated and caused cytopathological effects in both Aedes albopictus (C6/36) and Baby Hamster Syrian Kidney (BHK-21) cells. Agarose gel electrophoresis analysis revealed a viral genome comprised of 10 segments of double-stranded RNA, with a 1-2-2-1-1-1-1-1 migration pattern. Complete genome sequences of the JH2019C603 virus were determined through full-length cDNA amplification. Phylogenetic analysis based on the amino acid (aa) sequences of RNA-dependent RNA Polymerase (Pol), Major subcore (T2) and Major core-surface (T13) showed that JH2019C603 clustered with Yonaguni orbivirus (YONOV) from Japan, with aa identities relative to YONOV of 97.7% (Pol), 99.0% (T2) and 98.5% (T13). However, phylogenetic analysis based on the aa sequences of the outer capsid protein one and two (OC1 and OC2) showed that JH2019C603 formed an independent branch in the phylogenetic tree, and its aa identity with YONOV was only 55.4% (OC1) and 80.8% (OC2), respectively. Compared with the prototype of YONOV, a notable sequence deletion was observed in the 3' non-coding region of NS1, with the NS1 of JH2019C603 encoded within segment 7 (Seg-7), in contrast to YONOV, which contains NS1 in Seg-6. These results indicate that JH2019C603 belongs to the YONOV lineage and might be a novel serotype or a highly variant strain of YONOV. These findings will facilitate the identification of new isolates and clarify their geographical distribution, epidemiology, genetic diversity and possible disease associations.
2019年7月，在中国云南省亚热带地区的景洪市，从哨兵牛中分离出一种新型病毒株（JH2019C603）。该病毒在白纹伊蚊（C6/36）和叙利亚仓鼠肾细胞（BHK-21）中均能复制并引起细胞病变效应。琼脂糖凝胶电泳分析显示，病毒基因组由10个双链RNA片段组成，迁移模式为1-2-2-1-1-1-1-1。通过全长cDNA扩增确定了JH2019C603病毒的完整基因组序列。基于RNA依赖的RNA聚合酶（Pol）、主要亚核心（T2）和主要核心表面（T13）的氨基酸（aa）序列进行的系统发育分析表明，JH2019C603与来自日本的与那国环状病毒（YONOV）聚类，与YONOV的aa同一性分别为97.7%（Pol）、99.0%（T2）和98.5%（T13）。然而，基于外衣壳蛋白一和二（OC1和OC2）的aa序列进行的系统发育分析表明，JH2019C603在系统发育树中形成一个独立分支，其与YONOV的aa同一性分别仅为55.4%（OC1）和80.8%（OC2）。与YONOV原型相比，在NS1的3'非编码区观察到明显的序列缺失，JH2019C603的NS1编码在片段7（Seg-7）内，而YONOV的NS1包含在Seg-6中。这些结果表明，JH2019C603属于YONOV谱系，可能是YONOV的一种新型血清型或高度变异株。这些发现将有助于新分离株的鉴定，并阐明其地理分布、流行病学、遗传多样性和可能的疾病关联。
