Leavy Aisling, Jimenez-Mateos Eva M
Discipline of Physiology, School of Medicine, Trinity College Dublin, The University of Dublin, Green College, Dublin, Ireland.
Methods Mol Biol. 2023;2595:65-73. doi: 10.1007/978-1-0716-2823-2_4.
MicroRNAs are small molecules of non-coding RNAs involved in the regulation of mRNA expression, generally through inhibition of translation. Major efforts have been made to understand their role in health and disease, and more recently, microRNAs have been extensively studied as potential disease biomarkers. While the profiling and analysis of microRNAs from large quantities of biofluids are well established, difficulties still remain in the use of small volume of samples for this purpose. These include tissue samples for neurodevelopmental conditions, which may require the analysis of specific areas of the brain, e.g., hippocampus, or serum or plasma samples with a volume of fewer than 100 microliters. This chapter will give an overview of the preparation, profiling, and analysis of microRNAs from brain tissue with a starting mass of fewer than 100 micrograms.
微小RNA是一类非编码RNA小分子，通常通过抑制翻译来参与信使核糖核酸（mRNA）表达的调控。人们已做出大量努力来了解它们在健康和疾病中的作用，最近，微小RNA作为潜在的疾病生物标志物受到了广泛研究。虽然从大量生物流体中分析微小RNA的方法已经成熟，但使用少量样本进行此类分析仍存在困难。这些样本包括用于神经发育疾病研究的组织样本，这类样本可能需要分析大脑的特定区域，如海马体，或者体积小于100微升的血清或血浆样本。本章将概述起始质量少于100微克的脑组织中微小RNA的制备、分析及检测。
