核糖体蛋白S4与其16S结合位点S4-RNA形成的复合物的小角X射线滴定：30S亚基中的一个核心。
Small-angle X-ray titration of the complex formed between the ribosomal protein S4 and its 16S binding site, S4-RNA: a central core in the 30S subunit.
作者信息
Osterberg R, Sjöberg B, Garrett R A, Littlechild J
出版信息
Nucleic Acids Res. 1978 Oct;5(10):3579-87. doi: 10.1093/nar/5.10.3579.
Abstract
X-ray scattering titrations at 21 degree C and in ribosomal reconstitution buffer indicate that the S4-RNA and the protein S4 from a 1:1 complex with a stability constant, log K approximately 6.5. When the complex forms, there is only a limited change in the scattering curve indicating that S4-RNA essentially retains its conformation during the complex formation. The increase in the gyration radius as a result of the complex formation, delta R = 4 +/- 3 A, as well as the experimental scattering curve of the complex can be explained by models where the protein S4 is supposed to interact with the periphery of the S4-RNA.
摘要
在21摄氏度以及核糖体重构缓冲液中进行的X射线散射滴定表明，S4-RNA与蛋白质S4形成了1:1复合物，其稳定常数log K约为6.5。当复合物形成时，散射曲线仅有有限的变化，这表明S4-RNA在复合物形成过程中基本保持其构象。复合物形成导致的回转半径增加，ΔR = 4 ± 3 Å，以及复合物的实验散射曲线，可以通过蛋白质S4与S4-RNA外周相互作用的模型来解释。
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2
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3
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