Deutscher M P, Ghosh R K
Nucleic Acids Res. 1978 Oct;5(10):3821-9. doi: 10.1093/nar/5.10.3821.
Rabbit liver tRNA nucleotidyltransferase can be used to substitute nucleotides within the -C-C-A sequence of tRNA or to add nucleotides following this sequence. These anomolous reactions of the enzyme have been used to prepare radioactively-labeled synthetic tRNA precursors which mimic the structure of the natural precursors. Under appropriate conditions synthetic precursors of defined structure can be made. In this paper we describe the synthesis of tRNA-C-[14C]U and tRNA-C-C-A-[14C]C-C, which are representative of tRNA precursors containing altered residues within the -C-C-A sequence or with extra residues following the normal 3'terminus. A variety of other possible precursors can also be prepared. These synthetic tRNA precursors have already proved useful for isolation of possible tRNA processing nucleases.
兔肝tRNA核苷酸转移酶可用于替换tRNA的-C-C-A序列中的核苷酸，或在该序列之后添加核苷酸。该酶的这些异常反应已被用于制备模拟天然前体结构的放射性标记合成tRNA前体。在适当条件下，可以制备具有确定结构的合成前体。在本文中，我们描述了tRNA-C-[14C]U和tRNA-C-C-A-[14C]C-C的合成，它们分别代表在-C-C-A序列中含有改变的残基或在正常3'末端之后带有额外残基的tRNA前体。还可以制备多种其他可能的前体。这些合成tRNA前体已被证明可用于分离可能的tRNA加工核酸酶。
