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用tRNA核苷酸转移酶制备合成tRNA前体。

Preparation of synthetic tRNA precursors with tRNA nucleotidyltransferase.

作者信息

Deutscher M P, Ghosh R K

出版信息

Nucleic Acids Res. 1978 Oct;5(10):3821-9. doi: 10.1093/nar/5.10.3821.

DOI:10.1093/nar/5.10.3821
PMID:364418
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342712/
Abstract

Rabbit liver tRNA nucleotidyltransferase can be used to substitute nucleotides within the -C-C-A sequence of tRNA or to add nucleotides following this sequence. These anomolous reactions of the enzyme have been used to prepare radioactively-labeled synthetic tRNA precursors which mimic the structure of the natural precursors. Under appropriate conditions synthetic precursors of defined structure can be made. In this paper we describe the synthesis of tRNA-C-[14C]U and tRNA-C-C-A-[14C]C-C, which are representative of tRNA precursors containing altered residues within the -C-C-A sequence or with extra residues following the normal 3'terminus. A variety of other possible precursors can also be prepared. These synthetic tRNA precursors have already proved useful for isolation of possible tRNA processing nucleases.

摘要

兔肝tRNA核苷酸转移酶可用于替换tRNA的-C-C-A序列中的核苷酸，或在该序列之后添加核苷酸。该酶的这些异常反应已被用于制备模拟天然前体结构的放射性标记合成tRNA前体。在适当条件下，可以制备具有确定结构的合成前体。在本文中，我们描述了tRNA-C-[14C]U和tRNA-C-C-A-[14C]C-C的合成，它们分别代表在-C-C-A序列中含有改变的残基或在正常3'末端之后带有额外残基的tRNA前体。还可以制备多种其他可能的前体。这些合成tRNA前体已被证明可用于分离可能的tRNA加工核酸酶。

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本文引用的文献

1
Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳分离多种核糖核酸种类
Biochemistry. 1967 Jun;6(6):1818-27. doi: 10.1021/bi00858a033.
2
Reactions at the 3' terminus of transfer ribonucleic aid. IV. Extents of normal and anomalous nucleotide incorporation catalyzed by transfer ribonucleic acid nucleotidyltransferase.转移核糖核酸3'末端的反应。IV. 转移核糖核酸核苷酸转移酶催化的正常和异常核苷酸掺入程度。
J Biol Chem. 1972 Jan 25;247(2):469-80.
3
Reactions at the 3' terminus of transfer ribonucleic acid. 3. Catalytic properties of two purified rabbit liver transfer ribonucleic acid nucleotidyl transferases.转移核糖核酸3'末端的反应。3. 两种纯化的兔肝转移核糖核酸核苷酸转移酶的催化特性。
J Biol Chem. 1972 Jan 25;247(2):459-68.
4
Tyrosine tRNA precursor molecule polynucleotide sequence.酪氨酸转运核糖核酸前体分子多核苷酸序列。
Nat New Biol. 1971 Sep 8;233(36):35-9. doi: 10.1038/newbio233035a0.
5
Reactions at the 3' terminus of transfer ribonucleic acid. VII. Anomalous adenosine monophosphate incorporation catalyzed by rabbit liver transfer ribonucleic acid nucleotidyltransferase.
J Biol Chem. 1973 May 10;248(9):3116-21.
6
Reactions of the 3' terminus of transfer ribonucleic acid. VI. Properties of the poly(C) polymerase activity associated with rabbit liver transfer ribonucleic acid nucleotidyltransferase.
J Biol Chem. 1973 May 10;248(9):3108-15.
7
Isolation and partial characterization of Escherichia coli mutants with low levels of transfer ribonucleic acid nucleotidyltransferase.低水平转移核糖核酸核苷酸转移酶的大肠杆菌突变体的分离与部分特性分析
J Bacteriol. 1974 May;118(2):621-7. doi: 10.1128/jb.118.2.621-627.1974.
8
Mutants of Escherichia coli thermosensitive for the synthesis of transfer RNA.对转运RNA合成具有温度敏感性的大肠杆菌突变体。
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2091-5. doi: 10.1073/pnas.70.7.2091.
9
Purification and properties of a specific Escherichia coli ribonuclease which cleaves a tyrosine transfer ribonucleic acid presursor.一种可切割酪氨酸转移核糖核酸前体的特异性大肠杆菌核糖核酸酶的纯化及性质
J Biol Chem. 1972 Aug 25;247(16):5243-51.
10
Transfer RNA biosynthesis: the nucleotide sequence of a precursor to serine and proline transfer RNAs.转运RNA生物合成：丝氨酸和脯氨酸转运RNA前体的核苷酸序列
Proc Natl Acad Sci U S A. 1974 Feb;71(2):413-6. doi: 10.1073/pnas.71.2.413.

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