抗冻蛋白对冷冻鸡胸肉品质和微观结构的影响。
Effect of antifreeze protein on the quality and microstructure of frozen chicken breasts.
机构信息
Key Laboratory of Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Engineering Laboratory for Agro-products Processing, College of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China.
出版信息
Food Chem. 2023 Mar 15;404(Pt A):134555. doi: 10.1016/j.foodchem.2022.134555. Epub 2022 Oct 10.
The cryo-protective effects of different concentrations of antifreeze protein (AFP, 0 %, 0.10 %, 0.15 %, 0.20 %, 0.25 %, w/v) on chicken frozen at -18 °C for different storage perispeeods were investigated by monitoring water holding capacity (WHC), water distribution, color, protein properties, microstructure and histological changes. The results showed that AFP significantly maintained the quality traits of frozen chicken. Treatment with 0.20 % AFP represented highest cryo-protective effect on the WHC and protein properties during frozen storage. The carbonyl content with 0.20 % AFP was significantly lower than that of without AFP (P < 0.05). Besides, the myofibrils of chicken supplemented with AFP, especially with 0.25 % AFP, had significantly tighter microstructure, smaller ice crystal area and equivalent diameter than those of without AFP treatment (P < 0.05) after 75 d freezing storage. Overall, AFP had a positive effect to improve the quality of frozen chicken, and it was a potential, safe and efficient cryo-protective agent.
研究了不同浓度的抗冻蛋白（AFP，0%、0.10%、0.15%、0.20%、0.25%，w/v）对-18°C冷冻不同储存期鸡肉的冷冻保护作用，通过监测持水能力（WHC）、水分分布、颜色、蛋白质特性、微观结构和组织学变化。结果表明，AFP 能显著保持冷冻鸡肉的品质特性。在冷冻储存过程中，0.20% AFP 处理对 WHC 和蛋白质特性具有最高的冷冻保护作用。0.20% AFP 处理的羰基含量明显低于无 AFP 处理（P<0.05）。此外，添加 AFP 的鸡肉肌原纤维，尤其是添加 0.25% AFP 的肌原纤维，其微观结构明显更紧密，冰晶面积和等效直径明显小于无 AFP 处理（P<0.05），经过 75 天冷冻储存。总的来说，AFP 对改善冷冻鸡肉的品质有积极作用，是一种有潜力、安全、高效的冷冻保护剂。
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