Li Haijing, Wang Qian, Li Wenxin, Xia Xiufang
College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Foods. 2023 Sep 22;12(19):3530. doi: 10.3390/foods12193530.
Quality degradation due to the formation and growth of ice crystals caused by temperature fluctuations during storage, transportation, or retailing is a common problem in frozen surimi. While commercial antifreeze is used as an ingredient in frozen surimi, its high sweetness does not meet the contemporary consumer demand for low sugar and low calories. Therefore, the development of new green antifreeze agents to achieve an enhanced frozen-thawed stability of surimi has received more attention. The aim of this study was to develop a cryoprotectant (a mixture of citric acid and trehalose) to enhance the frozen-thawed stability of surimi by inhibiting the oxidative denaturation and structural changes of frozen-thawed mirror carp ( L.) surimi myofibrillar protein (MP). The results showed that the amounts of free amine, sulfhydryl, α-helix, intrinsic fluorescence intensity, and thermal stability in the control significantly decreased after five F-T cycles, while the Schiff base fluorescence intensity, amounts of disulfide bonds and surface hydrophobicity significantly increased ( < 0.05). Compared to sucrose + sorbitol (SS), the natural deep eutectic solvents (NADES) effectively inhibited protein oxidation. After five F-T cycles, the α-helix content and Ca-ATPase activity of the NADES samples were 4.32% and 80.0%, respectively, higher, and the carbonyl content was 17.4% lower than those of the control. These observations indicate that NADES could inhibit oxidative denaturation and enhance the structural stability of MP.
在冷冻鱼糜的储存、运输或零售过程中，由于温度波动导致冰晶形成和生长而引起的品质下降是一个常见问题。虽然商业抗冻剂被用作冷冻鱼糜的一种成分，但其高甜度不符合当代消费者对低糖和低热量的需求。因此，开发新型绿色抗冻剂以提高鱼糜的冻融稳定性受到了更多关注。本研究的目的是开发一种冷冻保护剂（柠檬酸和海藻糖的混合物），通过抑制冻融镜鲤鱼糜肌原纤维蛋白（MP）的氧化变性和结构变化来提高鱼糜的冻融稳定性。结果表明，在五个冻融循环后，对照组中游离胺、巯基、α-螺旋、内在荧光强度和热稳定性的含量显著降低，而席夫碱荧光强度、二硫键含量和表面疏水性显著增加（P<0.05）。与蔗糖+山梨醇（SS）相比，天然深共晶溶剂（NADES）能有效抑制蛋白质氧化。经过五个冻融循环后，NADES样品的α-螺旋含量和Ca-ATP酶活性分别比对照组高4.32%和80.0%，羰基含量比对照组低17.4%。这些观察结果表明，NADES可以抑制氧化变性并增强MP的结构稳定性。
