曼氏血吸虫感染的免疫诊断：用于检测循环抗原抗体的酶联免疫吸附测定
Immunodiagnosis of infection with Schistosoma mansoni: enzyme-linked immunosorbent assay for detection of antibody to circulating antigen.
作者信息
Kelsoe G H, Weller T H
出版信息
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5715-7. doi: 10.1073/pnas.75.11.5715.
Abstract
A circulating antigen, a negatively charged polysaccharide from the trematode Schistosoma mansoni, was noncovalently bound to the surface of poly(L-lysine)-coated wells in polystyrene trays, which were then used in a micro-enzyme-linked immunosorbent assay (ELISA) test. The method provides an immunodiagnostic test for schistosomiasis of exceptional sensitivity with a high degree of specificity. Comparison of Bell egg counts and ELISA titers revealed a good correlation (r congruent to 0.80) in young individuals with low to moderate worm burdens, but this relationship was less marked in older individuals or those with high egg counts.
摘要
一种循环抗原，即来自曼氏血吸虫的带负电荷的多糖，非共价结合于聚苯乙烯培养板中经聚（L - 赖氨酸）包被的孔表面，然后用于微酶联免疫吸附测定（ELISA）试验。该方法为血吸虫病提供了一种具有极高灵敏度和高度特异性的免疫诊断测试。对贝尔虫卵计数与ELISA滴度的比较显示，在虫负荷低至中等的年轻个体中存在良好的相关性（r约为0.80），但在年龄较大的个体或虫卵计数高的个体中这种关系不太明显。
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本文引用的文献
1
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Bull World Health Organ. 1963;29(4):525-30.
2
3
4
A perfusion procedure (perf-o-suction) for recovery of schistosome worms.一种用于回收血吸虫的灌注程序（灌注-抽吸法）。
J Parasitol. 1961 Jun;47:366-8.
5
Immunoelectrophoretic demonstration of specific circulating antigen in animals infected with Schistosoma mansoni.
Am J Trop Med Hyg. 1967 Sep;16(5):606-12. doi: 10.4269/ajtmh.1967.16.606.
6
A specific circulating antigen in hamsters infected with Schistosoma mansoni. Detection of antigen in serum and urine, and correlation between antigenic concentration and worm burden.感染曼氏血吸虫的仓鼠体内的一种特异性循环抗原。血清和尿液中抗原的检测以及抗原浓度与虫负荷之间的相关性。
Am J Trop Med Hyg. 1969 Jul;18(4):545-52. doi: 10.4269/ajtmh.1969.18.545.
7
An improved assay for haemolytic plaque-forming cells.一种改进的溶血空斑形成细胞检测方法。
Immunology. 1971 Feb;20(2):253-7.
8
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