酶标及¹²⁵I标记抗免疫球蛋白检测在血吸虫病免疫诊断中的应用
Enzyme- and 125I-labeled anti-immunoglobulin assays in the immunodiagnosis of schistosomiasis.
作者信息
Schinski V D, Clutter W C, Murrell K D
出版信息
Am J Trop Med Hyg. 1976 Nov;25(6):824-31. doi: 10.4269/ajtmh.1976.25.824.
An enzyme-linked immunosorbent assay (ELISA), and radioimmunoassays using antigen bound to plastic tubes or microcrystalline particles, were adapted for the indirect serological measurement of anti-schistosome antibodies. These alkaline phosphatase and 125I-labeled anti-immunoglobulin techniques were found to be highly quantitative and adaptable. Comparison showed them to be considerably more sensitive than the indirect hemagglutination and indirect fluorescent antibody techniques. Using adult S. mansoni freeze-thaw antigen, anti-schistosome antibodies were detected in sera from patients infected with Schistosoma mansoni and S. haematobium. However, results in patients with schistosomiasis haematobium were generally intermediate between those of patients with schistosomiasis mansoni and normal, uninfected controls.
酶联免疫吸附测定法（ELISA）以及使用结合在塑料管或微晶颗粒上的抗原的放射免疫测定法，被用于抗血吸虫抗体的间接血清学检测。发现这些碱性磷酸酶和¹²⁵I标记的抗免疫球蛋白技术具有高度定量性且适用性强。比较表明，它们比间接血凝和间接荧光抗体技术灵敏得多。使用曼氏血吸虫成虫冻融抗原，在感染曼氏血吸虫和埃及血吸虫的患者血清中检测到了抗血吸虫抗体。然而，埃及血吸虫病患者的检测结果通常介于曼氏血吸虫病患者和正常未感染对照之间。
Zotero 插件