另一种σ 因子σ 调控林肯链霉菌中林可霉素的产生。
An alternative σ factor σ regulates lincomycin production in Streptomyces lincolnensis.
机构信息
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Department of Applied Biology, East China University of Science and Technology, Shanghai, China.
出版信息
J Basic Microbiol. 2023 Feb;63(2):190-199. doi: 10.1002/jobm.202200485. Epub 2022 Dec 1.
Lincomycin produced by Streptomyces lincolnensis is a critical antibacterial antibiotic in the clinical. To further understand the regulatory mechanism of lincomycin biosynthesis, we identified an alternative σ factor, σ , in Streptomyces lincolnensis NRRL 2936. Deletion of sigL resulted in an increase in cell growth but a decrease in lincomycin production. σ boosted lincomycin biosynthesis by directly stimulating the transcription of four genes (lmbD, lmbV, lmrC, and lmbU) within the lincomycin biosynthetic lmb gene cluster. Besides, σ participated in lincomycin biosynthesis by directly stimulating the transcription of mshC, a gene responsible for MSH synthesis. In conclusion, our findings demonstrated that σ plays a direct regulatory role in lincomycin biosynthesis. This study extends the understanding of molecular mechanisms of lincomycin biosynthetic regulation.
林肯链霉菌产生的林可霉素是临床应用的重要抗菌抗生素。为了进一步了解林可霉素生物合成的调控机制，我们在林肯链霉菌 NRRL 2936 中鉴定出了一个替代σ因子，σ 。sigL 的缺失导致细胞生长增加，但林可霉素产量降低。σ 通过直接刺激林可霉素生物合成 lmb 基因簇内的四个基因（lmbD、lmbV、lmrC 和 lmbU）的转录来促进林可霉素生物合成。此外，σ 通过直接刺激负责 MSH 合成的 mshC 基因的转录参与林可霉素生物合成。总之，我们的研究结果表明，σ 在林可霉素生物合成中发挥直接调控作用。本研究扩展了对林可霉素生物合成调控的分子机制的理解。
Zotero 插件