State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Department of Applied Biology, East China University of Science and Technology, Shanghai, China.
J Basic Microbiol. 2023 Feb;63(2):190-199. doi: 10.1002/jobm.202200485. Epub 2022 Dec 1.
Lincomycin produced by Streptomyces lincolnensis is a critical antibacterial antibiotic in the clinical. To further understand the regulatory mechanism of lincomycin biosynthesis, we identified an alternative σ factor, σ , in Streptomyces lincolnensis NRRL 2936. Deletion of sigL resulted in an increase in cell growth but a decrease in lincomycin production. σ boosted lincomycin biosynthesis by directly stimulating the transcription of four genes (lmbD, lmbV, lmrC, and lmbU) within the lincomycin biosynthetic lmb gene cluster. Besides, σ participated in lincomycin biosynthesis by directly stimulating the transcription of mshC, a gene responsible for MSH synthesis. In conclusion, our findings demonstrated that σ plays a direct regulatory role in lincomycin biosynthesis. This study extends the understanding of molecular mechanisms of lincomycin biosynthetic regulation.
林肯链霉菌产生的林可霉素是临床应用的重要抗菌抗生素。为了进一步了解林可霉素生物合成的调控机制，我们在林肯链霉菌 NRRL 2936 中鉴定出了一个替代σ因子，σ 。sigL 的缺失导致细胞生长增加，但林可霉素产量降低。σ 通过直接刺激林可霉素生物合成 lmb 基因簇内的四个基因（lmbD、lmbV、lmrC 和 lmbU）的转录来促进林可霉素生物合成。此外，σ 通过直接刺激负责 MSH 合成的 mshC 基因的转录参与林可霉素生物合成。总之，我们的研究结果表明，σ 在林可霉素生物合成中发挥直接调控作用。本研究扩展了对林可霉素生物合成调控的分子机制的理解。
