Wu Xin, Wu Sheng-Bing, Zuo Hai-Yan, Wang Kun, Zhu Chao, Ruan Jing-Ru, Zeng Si-Yuan, Ge Ming-Xing, Cui Shuai, Zhou Mei-Qi
School of Acupuncture and Massage, Anhui University of Chinese Medicine, Hefei 230038, China.
Research and Technology Center, Anhui University of Chinese Medicine, Hefei 230038, China.
Zhen Ci Yan Jiu. 2022 Nov 25;47(11):962-8. doi: 10.13702/j.1000-0607.20210846.
To explore the effects of electroacupuncture(EA) on metabolic patterns of the prefrontal cortex in rats with acute myocardial ischemia.
Eighteen SD rats were randomly divided into sham group, model group and EA group, with 6 rats in each group. Rats in the model and EA groups were subject to acute myocardial ischemia by ligation of the left anterior descending coronary artery. For the EA group, EA stimulation (1 mA, 2 Hz/15 Hz, 30 min) was applied to "Shenmen"(HT7) -"Tongli"(HT5) once a day for 3 consecutive days. The histopathological changes of myocardial tissue and levels of ischemia modified albumin (IMA) in serum were determined by HE staining and ELISA, respectively. The LC-MS/MS technique was used to characterize the metabolic profiling of the prefrontal cortex. The differentially expressed metabolites were screened by principal component analysis(PCA) and partial least squares-linear discriminant analysis (PLS-LDA), and subsequently Kyoto encyclopedia of genes and genomes (KEGG) metabolic pathway enrichment analysis was performed.
Compared with the sham group, the myocardial fibers were disordered and fractured, and content of serum IMA was significantly increased in the model group (<0.01), which, however, were significantly decreased in the EA group (<0.01). With PCA and PLS-LDA, there were 18 differential metabolites between the model and sham groups. Forty-eight differential metabolites were emerged between the EA and model groups. Three metabolites associated to the sphingolipid metabolism were reversed by EA stimulation, as indicated by KEGG.
The molecular mechanism of EA against myocardial ischemia is partially mediated by regulating sphingolipid-related metabolites in the prefrontal cortex.
探讨电针（EA）对急性心肌缺血大鼠前额叶皮质代谢模式的影响。
将18只SD大鼠随机分为假手术组、模型组和电针组，每组6只。模型组和电针组大鼠通过结扎左冠状动脉前降支造成急性心肌缺血。电针组采用电针刺激（1 mA，2 Hz/15 Hz，30 min），每日1次，连续3天，取“神门”（HT7）-“通里”（HT5）穴位。分别采用HE染色和ELISA法检测心肌组织的病理组织学变化及血清缺血修饰白蛋白（IMA）水平。采用液相色谱-串联质谱（LC-MS/MS）技术对前额叶皮质的代谢谱进行表征。通过主成分分析（PCA）和偏最小二乘判别分析（PLS-LDA）筛选差异表达代谢物，随后进行京都基因与基因组百科全书（KEGG）代谢通路富集分析。
与假手术组相比，模型组心肌纤维紊乱、断裂，血清IMA含量显著升高（<0.01），而电针组显著降低（<0.01）。通过PCA和PLS-LDA分析，模型组与假手术组之间有18种差异代谢物。电针组与模型组之间出现48种差异代谢物。KEGG分析表明，电针刺激可逆转3种与鞘脂代谢相关的代谢物。
电针抗心肌缺血的分子机制部分是通过调节前额叶皮质中与鞘脂相关的代谢物来介导的。
