Department of Research & Innovation, Saveetha School of Engineering, SIMATS deemed University, Chennai 602195, Tamil Nadu, India.
Centre for Bioresource Research and Development (C-BIRD), Department of Biotechnology, Sathyabama Institute of Science and Technology, Chennai 600119, Tamil Nadu, India.
Int J Biol Macromol. 2023 Jan 31;226:1597-1610. doi: 10.1016/j.ijbiomac.2022.11.272. Epub 2022 Nov 28.
In this current work, the herbicidal activity of fungal metabolites stacked chitosan nanocomposite against significant aquatic invasive weed Eichhornia crassipes (water hyacinth) was examined. Herbicidal metabolites from the fungal strain Allophoma oligotrophica were extracted and purified under standard condition. Altered ionic gelation technique was received for the amalgamation of chitosan nanocomposite fabricated with herbicidal metabolites. Synthesized nanocomposite incited checked herbicidal impact on the leaflets of water hyacinth. Synthesized nanocomposite induced marked herbicidal effect on the tested leaflets of water hyacinth. Necrotic development on the tested leaflets at earlier incubation period followed by progressive enhancement of necrotic lesion reveals the noteworthy herbicidal activity of the synthesized nanocomposite. Parenchymal, mesenchymal tissue disintegration, reduction of total chlorophyll content, elevated anti oxidative enzymes and changes in qualitative protein profiling of tested leaflets reveals the nanocomposite induced noteworthy morphometric and functional effects. Effect of solvents on the release profile demonstrates that ethyl acetate treatment brought about controlled or sustained release of metabolites. No sign of toxic effect on the zebra fish embryonic developmental stages revealed biocompatibility of the nanocomposite.
在当前这项工作中,研究了真菌代谢物堆叠壳聚糖纳米复合材料对重要的水生入侵杂草凤眼莲(水葫芦)的除草活性。在标准条件下提取并纯化了来自真菌菌株寡营养异担子菌的除草代谢物。采用改进的离子凝胶技术将除草代谢物制备的壳聚糖纳米复合材料进行复合。合成的纳米复合材料对水葫芦小叶产生了显著的除草作用。合成的纳米复合材料对测试的水葫芦小叶产生了明显的除草效果。在早期培养阶段,测试小叶上出现坏死发展,随后坏死病变逐渐加重,这表明合成的纳米复合材料具有显著的除草活性。测试小叶的实质、间质组织解体、总叶绿素含量降低、抗氧化酶升高以及定性蛋白质谱变化表明,纳米复合材料诱导了显著的形态计量学和功能效应。溶剂对释放曲线的影响表明,乙酸乙酯处理导致代谢物的控制释放或持续释放。对斑马鱼胚胎发育阶段没有毒性作用迹象,表明纳米复合材料具有生物相容性。