College of Landscape Architecture and Horticulture Sciences, Southwest Research Center for Engineering Technology of Landscape Architecture (State Forestry and Grassland Administration), Yunnan Engineering Research Center for Functional Flower Resources and Industrialization, Research and Development Center of Landscape Plants and Horticulture Flowers, Southwest Forestry University, Kunming, 650224, Yunnan, China.
Key Laboratory for Plant Biodiversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China.
BMC Plant Biol. 2022 Dec 1;22(1):553. doi: 10.1186/s12870-022-03894-1.
Spur, a structure capable of producing and storing nectar, not only plays a vital role in the pollination process but also promotes the rapid diversification of some plant lineages, which is considered a key innovation in plants. Spur is the focus of many studies, such as evolution and ecological hypothesis, but the current understanding of spur development is limited. High-throughput sequencing of Impatiens uliginosa was carried out to study the molecular mechanism of its spur development, which is believed to provide some insights into the spur development of Impatiens.
Transcriptomic sequencing and analysis were performed on spurs and limbs of I. uliginosa at three developmental stages. A total of 47.83 Gb of clean data were obtained, and 49,716 unigene genes were assembled. After comparison with NR, Swiss-Prot, Pfam, COG, GO and KEGG databases, a total of 27,686 genes were annotated successfully. Through comparative analysis, 19,356 differentially expressed genes were found and enriched into 208 GO terms and 146 KEGG pathways, among which plant hormone signal transduction was the most significantly enriched pathway. One thousand thirty-two transcription factors were identified, which belonged to 33 TF families such as MYB, bHLH and TCP. Twenty candidate genes that may be involved in spur development were screened and verified by qPCR, such as SBP, IAA and ABP.
Transcriptome data of different developmental stages of spurs were obtained, and a series of candidate genes related to spur development were identified. The importance of genes related to cell cycle, cell division, cell elongation and hormones in spur development was clarified. This study provided valuable information and resources for understanding the molecular mechanism of spur development in Impatiens.
距，一种能够产生和储存花蜜的结构，不仅在授粉过程中起着至关重要的作用，而且促进了一些植物谱系的快速多样化，这被认为是植物的一个关键创新。距是许多研究的焦点，如进化和生态假说，但目前对距发育的理解有限。对延胡索进行高通量测序，以研究其距发育的分子机制，这有望为延胡索距发育提供一些见解。
对三个发育阶段的延胡索距和肢体进行了转录组测序和分析。共获得 47.83 Gb 的清洁数据，组装了 49716 条 unigene 基因。与 NR、Swiss-Prot、Pfam、COG、GO 和 KEGG 数据库进行比较后，共成功注释了 27686 个基因。通过比较分析，发现了 19356 个差异表达基因，并富集到 208 个 GO 术语和 146 个 KEGG 途径，其中植物激素信号转导是最显著富集的途径。鉴定出 1032 个转录因子，属于 MYB、bHLH 和 TCP 等 33 个 TF 家族。通过 qPCR 筛选和验证了 20 个可能参与距发育的候选基因，如 SBP、IAA 和 ABP。
获得了不同发育阶段距的转录组数据，鉴定出一系列与距发育相关的候选基因。阐明了与细胞周期、细胞分裂、细胞伸长和激素相关的基因在距发育中的重要性。本研究为理解延胡索距发育的分子机制提供了有价值的信息和资源。
